Anti-Proinsulin antibody [3B6-19-1]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Proinsulin antibody [3B6-19-1] (AB243141)

Intracellular flow cytometric analysis of 2% PFA-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cells (PBMC) (right) labeling Proinsulin with ab243141 at 0.98μg/mL compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab243141 (Right). Then stained with anti-CD14 (Alexa Fluor^® 647).

Gated on viable cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] (AB243141)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Proinsulin with ab243141 at 0.49 μg/mL, followed by a ready to use secondary. Positive staining on islets in the human pancreas is observed. The section was incubated with ab243141 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument with no antigen retrieval. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] (AB243141)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Proinsulin with ab243141 at 0.49 μg/mL, followed by a ready to use secondary. Positive staining on islets in the rat pancreas is observed. The section was incubated with ab243141 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument with no antigen retrieval. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proinsulin antibody [3B6-19-1] (AB243141)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Proinsulin with ab243141 at 0.49 μg/mL, followed by a ready to use secondary. Positive staining on islets in the mouse pancreas is observed. The section was incubated with ab243141 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument with no antigen retrieval. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary.

• Dot

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Dot Blot - Anti-Proinsulin antibody [3B6-19-1] (AB243141)

Dot Blot - Anti-Proinsulin antibody [3B6-19-1] (ab243141, 0.49 μg/mL).

Secondary used is a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution.

Sample is an insulin recombinant protein.

Blocking/Dilution buffer : 5% NFDM/TBST.