AB244829

Anti-Proinsulin antibody [EPR20432-54] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Insulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Insulin, INS

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Sandwich ELISA - Anti-Proinsulin antibody [EPR20432-54] - BSA and Azide free (Capture) (AB244829)

sELISA

Unknown

Sandwich ELISA - Anti-Proinsulin antibody [EPR20432-54] - BSA and Azide free (Capture) (AB244829)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab242235).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20432-54

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab244829 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Proinsulin. The recommended pair for sandwich ELISA is:
Capture: ab244829, Proinsulin Capture Antibody (unconjugated)
Detector: ab244849, Proinsulin Detector Antibody (unconjugated)
The reference range value is 46.88 - 3000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proinsulin is a precursor molecule important for insulin production. Its alternate names include 'INS-IGF2' or 'preproinsulin'. It has a molecular mass of approximately 9 kDa. The primary expression occurs in the beta cells of the pancreatic islets. Proinsulin undergoes enzymatic cleavage to produce insulin and C-peptide executing a significant transformation as it matures into active insulin which plays an important role in glucose metabolism.

Biological function summary

Proinsulin facilitates the synthesis and release of insulin an essential process involving the conversion from proinsulin to insulin. Proinsulin itself does not operate as part of a complex but requires processing enzymes like prohormone convertase to mature into functional insulin. The efficient conversion of proinsulin to insulin allows cells to maintain glucose homeostasis highlighting its importance in cellular energy usage.

Pathways

Insulin biosynthesis and secretion and the glucose homeostasis pathway rely heavily on proinsulin. In the insulin biosynthesis pathway proinsulin acts upstream of insulin the hormone directly regulating blood glucose levels. The pathways involve interaction with proteins such as proprotein convertase subtilisin/kexin type 1 (PCSK1) which participates in the conversion of proinsulin to insulin. Through these pathways proinsulin ensures proper energy regulation and balances the body's glucose supply with cellular demands.

Aberrations in proinsulin processing or levels closely associate with diabetes mellitus and hyperinsulinemia. Diabetes mellitus characterized by insulin deficiency or resistance can result from insufficient proinsulin conversion to insulin. Hyperinsulinemia involves excess levels of insulin-related peptides sometimes due to incomplete proinsulin processing. Disorders affecting proinsulin often involve its relationship with insulin where inadequate or excess production directly influences blood glucose regulation and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information INS

Additional targets

Proinsulin

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proinsulin antibody [EPR20432-54] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Proinsulin antibody [EPR20432-54] - BSA and Azide free (Capture) (AB244829)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise