AB103617

Anti-Prolactin/PRL antibody

Name: Anti-Prolactin/PRL antibody (ab103617) | Abcam
Brand: Abcam Limited
SKU: AB103617
Price: 490 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Prolactin/PRL antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Recombinant fragment - Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRL.

View Alternative Names

Prolactin, PRL

4 Images

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Western blot - Anti-Prolactin/PRL antibody (AB103617)

All lanes:

Western blot - Anti-Prolactin/PRL antibody (ab103617) at 1/500 dilution

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Mouse testis tissue lysate at 50 µg

Predicted band size: 26 kDa

false

Unknown

Western blot - Anti-Prolactin/PRL antibody (AB103617)

All lanes:

Western blot - Anti-Prolactin/PRL antibody (ab103617) at 1/500 dilution

Lane 1:

Prolactin/PRL transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 26 kDa

false

Unknown

Western blot - Anti-Prolactin/PRL antibody (AB103617)

All lanes:

Western blot - Anti-Prolactin/PRL antibody (ab103617) at 1/500 dilution

All lanes:

Western blot - Recombinant human Prolactin/PRL protein (<a href='/en-us/products/proteins-peptides/recombinant-human-prolactin-prl-protein-ab51703'>ab51703</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 26 kDa

true

Exposure time: 90s

Unknown

Western blot - Anti-Prolactin/PRL antibody (AB103617)

All lanes:

Western blot - Anti-Prolactin/PRL antibody (ab103617) at 1/500 dilution

All lanes:

Western blot - Recombinant human Prolactin/PRL protein (<a href='/en-us/products/proteins-peptides/recombinant-human-prolactin-prl-protein-ab51703'>ab51703</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 26 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRL.

P01236

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.

Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Prolactin acts primarily on the mammary gland by promoting lactation.

See full target information PRL

Additional targets

Prolactin/PRL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 622:627-636 PubMed37821702

2023

Apoptotic stress causes mtDNA release during senescence and drives the SASP.

Applications

Unspecified application

Species

Unspecified reactive species

Stella Victorelli,Hanna Salmonowicz,James Chapman,Helene Martini,Maria Grazia Vizioli,Joel S Riley,Catherine Cloix,Ella Hall-Younger,Jair Machado Espindola-Netto,Diana Jurk,Anthony B Lagnado,Lilian Sales Gomez,Joshua N Farr,Dominik Saul,Rebecca Reed,George Kelly,Madeline Eppard,Laura C Greaves,Zhixun Dou,Nicholas Pirius,Karolina Szczepanowska,Rebecca A Porritt,Huijie Huang,Timothy Y Huang,Derek A Mann,Claudio Akio Masuda,Sundeep Khosla,Haiming Dai,Scott H Kaufmann,Emmanouil Zacharioudakis,Evripidis Gavathiotis,Nathan K LeBrasseur,Xue Lei,Alva G Sainz,Viktor I Korolchuk,Peter D Adams,Gerald S Shadel,Stephen W G Tait,João F Passos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prolactin/PRL antibody

Western blot - Anti-Prolactin/PRL antibody (AB103617)

Western blot - Anti-Prolactin/PRL antibody (AB103617)

Western blot - Anti-Prolactin/PRL antibody (AB103617)

Western blot - Anti-Prolactin/PRL antibody (AB103617)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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