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Rabbit Recombinant Monoclonal Prolactin/PRL antibody. Suitable for IHC-Fr, WB, IHC-P, IP and reacts with Mouse, Rat samples. Cited in 4 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229), expandable thumbnail
  • Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229), expandable thumbnail
  • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (AB188229), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrWBIHC-PIP
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol.

Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/16000
Notes

This antibody is not suitable for human species in IHC application.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/16000
Notes

This antibody is not suitable for human species in IHC application.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

12 products for Alternative Product

Target data

Function

Prolactin acts primarily on the mammary gland by promoting lactation.

Additional Targets

Prolactin/PRL

Alternative names

Prolactin, PRL, Prl

Recommended products

Rabbit Recombinant Monoclonal Prolactin/PRL antibody. Suitable for IHC-Fr, WB, IHC-P, IP and reacts with Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18018-31
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.

Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

Associated diseases and disorders

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse pituitary (PMID: 24514953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229), expandable thumbnail

    Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Blocking/Dilution buffer: 5% NFDM/TBST

    Prolactin expression is generally restricted to the anterior pituitary gland (PMID: 1697858).

    Negative control: Mouse kidney, rat heart and C6 cells.

    All lanes: Western blot - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229) at 1/5000 dilution

    Lane 1: Rat pituitary tissue lysate at 20 µg

    Lane 2: Mouse pituitary tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Rat heart tissue lysate at 20 µg

    Lane 5: C6 (rat glial tumor cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 23 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pituitary (PMID: 24514953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229), expandable thumbnail

    Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary tissue lysate with ab188229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse pituitary tissue lysate 10 μg (Input).

    Lane 2: ab188229 IP in mouse pituitary tissue lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab188229 in mouse pituitary tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Predicted band size: 26 kDa

    Observed band size: 23 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] (ab188229)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Cytoplasmic staining in lactotrophic cells of anterior pituitary gland (PMID: 16468032). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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