Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free (AB250714)

This data was developed using ab183968, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed negative staining on mouse kidney.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free (AB250714)

This data was developed using ab183968, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse anterior pituitary.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free (AB250714)

This data was developed using ab183968, the same antibody clone in a different buffer formulation.

Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary whole cell lysate with ab183968 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183968 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse pituitary whole cell lysate, 10μg (Input).
Lane 2 : ab183968 IP in Mouse pituitary whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183968 in Mouse pituitary whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (<a href='/en-us/products/primary-antibodies/prolactin-prl-antibody-epr19372-ab183968'>ab183968</a>)

Predicted band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-Prolactin/PRL antibody [EPR19372] - BSA and Azide free (AB250714)

This data was developed using ab183968, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 8 seconds; Lanes 2-6 : 3 second.

All lanes:

Western blot - Anti-Prolactin/PRL antibody [EPR19372] (<a href='/en-us/products/primary-antibodies/prolactin-prl-antibody-epr19372-ab183968'>ab183968</a>) at 1/5000 dilution

Lane 1:

Mouse pituitary lysate at 20 µg

Lane 2:

Rat pituitary lysate at 20 µg

Lane 3:

Mouse uterus tissue lysate at 20 µg

Lane 4:

Rat kidney tissue lysate at 20 µg

Lane 5:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 25 kDa

false