AB183967

Anti-Prolactin/PRL antibody [EPR19386]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal Prolactin/PRL antibody. Suitable for IHC-Fr, ICC/IF, IHC-P, WB and reacts with Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Prolactin, PRL, Prl, Decidual prolactin, GHA1, Growth hormone A1, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, Prolactin, Prolactin precursor

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Prolactin/PRL with ab183967 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The results show positive staining on HeLa cells transfected with myc-tagged Prolactin.

The nuclear counter stain is DAPI (blue).

Myc is detected with anti-c-Myc (mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab183967 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : Anti-c-Myc at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

The results show positive staining on HeLa cells transfected with myc-tagged Prolactin.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab183967 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183967 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse anterior pituitary.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Prolactin/PRL with ab183967 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining was observed on mouse spleen.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183967 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

No staining was observed on mouse kidney.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemical analysis of paraffin-embedded Mouse adenohypophysis tissue labeling Prolactin/PRL with ab183967 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse adenohypophysis was observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Prolactin/PRL with ab183967 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining was observed on mouse stomach.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1, 2, 3, 4 and 5 : 5 seconds; Lane 6 : 1 second.

All lanes:

Western blot - Anti-Prolactin/PRL antibody [EPR19386] (ab183967) at 1/1000 dilution

Lane 1:

Mouse pituitary lysate at 20 µg

Lane 2:

Mouse uterus lysate at 20 µg

Lane 3:

Rat heart lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 6:

Rat pituitary lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 25 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Prolactin/PRL antibody [EPR19386]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Prolactin/PRL antibody [EPR19386]
Carrier free

Anti-Prolactin/PRL antibody [EPR19386] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19386

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB, ICC/IF, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "Perform antigen retrieval with heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.

Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Prolactin acts primarily on the mammary gland by promoting lactation.

See full target information Prl

Additional targets

Prolactin/PRL

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Neuroendocrinology 114:517-537 PubMed38071956

2023

Oxytocin Receptor-Expressing Neurons in the Medial Preoptic Area Are Essential for Lactation, whereas Those in the Lateral Septum Are Not Critical for Maternal Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Shizu Hidema,Keisuke Sato,Hiroaki Mizukami,Yumi Takahashi,Yuko Maejima,Kenju Shimomura,Katsuhiko Nishimori

International journal of molecular sciences 23: PubMed36555554

2022

TRH Regulates the Synthesis and Secretion of Prolactin in Rats with Adenohypophysis through the Differential Expression of miR-126a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Kun Zhao,Yi Zheng,Hai-Xiang Guo,Hao-Qi Wang,Zhong-Hao Ji,Tian Wang,Song Yu,Jia-Bao Zhang,Bao Yuan,Wen-Zhi Ren

BMC biology 20:276 PubMed36482461

2022

An IGF1-expressing endometrial stromal cell population is associated with human decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Wei Shi,Zhen-Zhen Lai,Hui-Li Yang,Wen-Jie Zhou,Xiao-Ya Zhao,Feng Xie,Song-Ping Liu,Wei-Dong Chen,Tao Zhang,Jiang-Feng Ye,Xiang-Yu Zhou,Ming-Qing Li

Pathology oncology research : POR 27:1609761 PubMed34257616

2021

MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Wang,Dan Liu,Jun Wei,Liwei Yuan,Shiyun Zhao,Yani Huang,Jingwen Ma,Zhijuan Yang

Molecular biology and evolution 37:2679-2690 PubMed32421768

2020

Molecular Signatures of Placentation and Secretion Uncovered in Poeciliopsis Maternal Follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Michael W Guernsey,Henri van Kruistum,David N Reznick,Bart J A Pollux,Julie C Baker

Oncology letters 11:1829-1834 PubMed26998085

2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zengjin Liu,Huamin Han,Xin He,Shouwei Li,Chenxing Wu,Chunjiang Yu,Shengdian Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prolactin/PRL antibody [EPR19386]

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

Western blot - Anti-Prolactin/PRL antibody [EPR19386] (AB183967)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (6)

Product promise