AB214303

Anti-Prolactin Receptor/PRL-R antibody

Name: Anti-Prolactin Receptor/PRL-R antibody (ab214303) | Abcam
Brand: Abcam Limited
SKU: AB214303
Price: 485 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(5 Publications)

Rabbit Polyclonal Prolactin Receptor/PRL-R antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PRLR aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Prolactin receptor, PRL-R, PRLR

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

Immunohistochemical analysis of formalin-fixed and paraffin embedded rat pancreas tissue labeling Prolactin Receptor/PRL-R using ab214303 at 1/200, followed by secondary detection and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse pancreas tissue labeling Prolactin Receptor/PRL-R using ab214303 at 1/400, followed by secondary detection and DAB staining. Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Blocked endogenous peroxidase with 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min;

Supplier Data

Western blot - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

All lanes:

Western blot - Anti-Prolactin Receptor/PRL-R antibody (ab214303) at 1/300 dilution

All lanes:

mouse brain lysate

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PRLR aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P16471

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.

Biological function summary

The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.

Pathways

PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.

Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

See full target information PRLR

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113112 PubMed40792031

2025

A long-lasting prolactin stimulates galactopoiesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kasia Kready,Kailyn E Doiron,Katherine Redfield Chan,Jeffrey C Way,Quincey Justman,Camille E Powe,Pamela A Silver

Cell death discovery 10:12 PubMed38184644

2024

Fatty acid amide hydrolase drives adult mammary gland development by promoting luminal cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Tundidor,Marta Seijo-Vila,Sandra Blasco-Benito,María Rubert-Hernández,Gema Moreno-Bueno,Laura Bindila,Rubén Fernández de la Rosa,Manuel Guzmán,Cristina Sánchez,Eduardo Pérez-Gómez

Neuroendocrinology 114:517-537 PubMed38071956

2023

Oxytocin Receptor-Expressing Neurons in the Medial Preoptic Area Are Essential for Lactation, whereas Those in the Lateral Septum Are Not Critical for Maternal Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Shizu Hidema,Keisuke Sato,Hiroaki Mizukami,Yumi Takahashi,Yuko Maejima,Kenju Shimomura,Katsuhiko Nishimori

Cellular oncology (Dordrecht, Netherlands) 45:1217-1236 PubMed36374443

2022

CEP55 3'-UTR promotes epithelial-mesenchymal transition and enhances tumorigenicity of bladder cancer cells by acting as a ceRNA regulating miR-497-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglin Yang,Yue Yang,Wei Wang,Wuer Zhou,Xiaoming Zhang,Yuansong Xiao,Huifen Zhang

Journal of neuroendocrinology 31:e12783 PubMed31433881

2019

Fatherhood diminishes the hippocampal damaging action of excitotoxic lesioning in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ilektra Anagnostou,Teresa Morales

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prolactin Receptor/PRL-R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

Western blot - Anti-Prolactin Receptor/PRL-R antibody (AB214303)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise