Mouse Monoclonal Prolactin Receptor/PRL-R antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human PRLR.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2] (AB233958), expandable thumbnail](https://content.abcam.com/products/images/prolactin-receptor-prl-r-antibody-b62-ab233958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2] (AB233958)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human PRLR. Database link P16471
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Alternative names
Prolactin receptor, PRL-R, PRLR
Recommended products
Mouse Monoclonal Prolactin Receptor/PRL-R antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human PRLR.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human PRLR. Database link P16471
- Clone number
- B6.2
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.
- Biological function summary
The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.
- Pathways
PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.
- Associated diseases and disorders
Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2] (ab233958), expandable thumbnail](https://content.abcam.com/products/images/prolactin-receptor-prl-r-antibody-b62-ab233958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62730.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2] (ab233958)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2] (ab233958)
Formalin-fixed, paraffin-embedded human testicular cancer tissue stained for Prolactin Receptor/PRL-R using ab233958 at 1 μg/ml in immunohistochemical analysis.
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