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AB245067

Anti-Prolactin Receptor/PRL-R antibody [EPR21740-239] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Prolactin Receptor/PRL-R antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

View Alternative Names

Prolactin receptor, PRL-R, Lactogen receptor, Prlr

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Sandwich ELISA - Anti-Prolactin Receptor/PRL-R antibody [EPR21740-239] - BSA and Azide free (Detector) (AB245067)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Prolactin Receptor/PRL-R antibody [EPR21740-239] - BSA and Azide free (Detector) (AB245067)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab238259).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21740-239

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab245067 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat Prolactin Receptor / PRL-R. The recommended pair for sandwich ELISA is:
Capture: ab244794, Rat Prolactin Receptor / PRL-R Capture Antibody (unconjugated)
Detector: ab245067, Rat Prolactin Receptor / PRL-R Detector Antibody (unconjugated)
The reference range value is 12.5 - 800 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.
Biological function summary

The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.

Pathways

PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.

Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is a receptor for the anterior pituitary hormone prolactin.
See full target information Prlr
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Complementary Products

Assay Kits

AB238259

Rat Prolactin Receptor / PRL-R ELISA Kit

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Sandwich ELISA - Rat Prolactin Receptor / PRL-R ELISA Kit (AB238259)

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com