AB2773

Anti-Prolactin Receptor/PRL-R antibody [T6]

Name: Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773) | Abcam
Brand: Abcam Limited
SKU: AB2773
Price: 520 USD
Availability: InStock
Rating: 1 (2 reviews)

(2 Reviews)

(8 Publications)

Mouse Monoclonal Prolactin Receptor/PRL-R antibody. Suitable for IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 8 publications.

View Alternative Names

Prolactin receptor, PRL-R, PRLR

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (AB2773)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (AB2773)

Immunocytochemical analysis of C6 (Rat glial tumor cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1 : 200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (AB2773)

Immunocytochemical analysis of H-4-II-E (Rat hepatoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1 : 200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (AB2773)

Immunocytochemical analysis of in SW480 (Human colorectal adenocarcinoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1 : 200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (AB2773)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (AB2773)

Immunohistochemical analysis of normal biopsies of deparaffinized rat pituitary gland tissue labeling Prolactin Receptor/PRL-R. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1 : 50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2a

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P

applications

Specificity

Detects Prolactin Receptor/PRL-R in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing Prolactin Receptor/PRL-R in NB2 cell lysate. Immunohistochemical staining of Prolactin Receptor/PRL-R in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to Prolactin Receptor/PRL-R in vitro.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" } } }

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AB309317

Human Prolactin Receptor/PRL-R ELISA Kit

0 Reviews

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.

Biological function summary

The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.

Pathways

PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.

Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

See full target information PRLR

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37958809

2023

mRNA and Protein Expression in Human Fetal Membrane Cells: Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes?

Applications

Unspecified application

Species

Unspecified reactive species

Emmeli Mikkelsen,Berthold Huppertz,Ripudaman Singh,Katarina Ravn,Lotte Hatt,Mogens Kruhøffer,Rheanna Urrabaz-Garza,Niels Uldbjerg,Ramkumar Menon,Torben Steiniche

Frontiers in physiology 13:778116 PubMed35309064

2022

The Circ-CYP24A1-miR-224-PRLR Axis Impairs Cell Proliferation and Apoptosis in Recurrent Miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Su,Jiani Xu,Rufei Gao,Xiaoli Liu,Taihang Liu,Cong Li,Yubin Ding,Xuemei Chen,Junlin He,Xueqing Liu,Chunli Li,Hongbo Qi,Yingxiong Wang

Endocrinology 135:1299-306 PubMed7925093

1994

JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization.

Applications

Unspecified application

Species

Unspecified reactive species

H Rui,J J Lebrun,R A Kirken,P A Kelly,W L Farrar

Endocrinology 134:2108-14 PubMed8156910

1994

Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry.

Applications

Unspecified application

Species

Unspecified reactive species

M Dardenne,M do C de Moraes,P A Kelly,M C Gagnerault

Journal of immunology (Baltimore, Md. : 1950) 150:5673-81 PubMed8515082

1993

Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.

Applications

Unspecified application

Species

Unspecified reactive species

M C Gagnerault,P Touraine,W Savino,P A Kelly,M Dardenne

Proceedings of the National Academy of Sciences of the United States of America 88:9700-4 PubMed1946390

1991

Identification and functional activity of prolactin receptors in thymic epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

M Dardenne,P A Kelly,J F Bach,W Savino

The Journal of biological chemistry 266:16472-7 PubMed1885580

1991

Mutational analysis of the ligand-binding domain of the prolactin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

M Rozakis-Adcock,P A Kelly

Endocrinology 124:2499-508 PubMed2707162

1989

Characterization and applications of monoclonal antibodies to the prolactin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

H Okamura,J Zachwieja,S Raguet,P A Kelly

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

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Alternative Product

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