AB2772

Anti-Prolactin Receptor/PRL-R antibody [U5]

Name: Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772) | Abcam
Brand: Abcam Limited
SKU: AB2772
Price: 510 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(26 Publications)

Mouse Monoclonal Prolactin Receptor/PRL-R antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF, FuncS (Neut/Block), IHC-Fr and reacts with Rat, Pig, Human, Horse, Sheep, Rabbit samples. Cited in 26 publications.

View Alternative Names

Prolactin receptor, PRL-R, PRLR

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in HepG2 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1 : 20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in MCF-7 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1 : 20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

IHC-P

AbReview20492****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

ab2772 staining the Prolactin Receptor/PRL-R in Human salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Flow Cyt

Unknown

Flow Cytometry - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Overlay histogram showing MCF-7 cells stained with ab2772 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2772, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in A2058 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1 : 20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Mammary gland tissue from lactating light horse mare was incubated with ab2772 at 1 : 100 for 1 hour at 23°C and for 24 hours at 4°C. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody (23°C for 1 hour) .Development using avidin-biotin complex 23°C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin. Antigen retrieval was facilitated using steaming at 95°C for 20 min in Trizma® buffer (pH 10.0) . Blocking endogenous peroxidase performed with 0.3% H2O2 and MeOH (20 min at 23°C). To decrease non-specific staining, tissue was pre-incubated in normal goat serum for 30 min at 23°C.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Reacts with

Horse, Human, Rat, Rabbit, Sheep, Pig

Applications

IP, IHC-P, ICC/IF, IHC-Fr, WB, Flow Cyt, FuncS (Neut/Block)

applications

Epitope

This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.

Specificity

Detects prolactin (PRL) receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. ab2772, after biotinylation, has been used to detect Human lymphocyte PRL receptor by FACS. ab2772 does not inhibit the interaction of prolactin with the receptor.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. ab2772, after biotinylation, has been used to detect Human lymphocyte PRL receptor by FACS. ab2772 does not inhibit the interaction of prolactin with the receptor.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Horse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. ab2772, after biotinylation, has been used to detect Human lymphocyte PRL receptor by FACS. ab2772 does not inhibit the interaction of prolactin with the receptor.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. ab2772, after biotinylation, has been used to detect Human lymphocyte PRL receptor by FACS. ab2772 does not inhibit the interaction of prolactin with the receptor.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "predicted", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. ab2772, after biotinylation, has been used to detect Human lymphocyte PRL receptor by FACS. ab2772 does not inhibit the interaction of prolactin with the receptor.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. ab2772, after biotinylation, has been used to detect Human lymphocyte PRL receptor by FACS. ab2772 does not inhibit the interaction of prolactin with the receptor.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin receptor (PRL-R) also known as PRL receptor is a protein that binds prolactin a hormone essential for lactation and various other biological processes. PRL-R is a member of the type I cytokine receptor family and is located on cell surfaces. It has several isoforms including the full form and the delta 2772 variant. The receptor weighs approximately 80 kDa and is widely expressed in tissues such as the mammary gland liver and immune cells.

Biological function summary

The prolactin receptor plays a significant role in regulating lactation reproductive and immunological functions. It binds prolactin to form a ligand-receptor complex initiating signal transduction pathways within cells. PRL-R is not part of a larger complex but instead activates intracellular signaling cascades leading to various cellular responses including growth and differentiation. This receptor is important for normal breast development and differentiation during pregnancy and lactation phases.

Pathways

PRL-R participates in the JAK2-STAT5 and PI3K-AKT pathways. These pathways are vital for mediating the biological effects of prolactin on cell division and survival. The JAK2-STAT5 pathway facilitates the transcription of genes involved in growth and proliferation while the PI3K-AKT pathway is important for metabolism and cell survival. PRL-R functionally interacts with proteins such as JAK2 which phosphorylates STAT5 propagating the signal initiated by receptor engagement.

Prolactin receptor abnormalities can be tied to breast cancer and prolactinoma. Dysregulation of PRL-R through overexpression or mutations can lead to uncontrolled cell growth and contribute to cancer development particularly in breast tissue. Prolactinoma a pituitary gland tumor leads to excessive production of prolactin exacerbating PRL-R activity. In these contexts proteins like estrogen receptor in breast cancer and pituitary tumor transforming gene (PTTG) in prolactinoma may be intertwined with prolactin receptor-related disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

See full target information PRLR

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Cephalalgia : an international journal of headache 45:3331024241313378 PubMed39814523

2025

Prolactin-induced sensitization of trigeminal nociceptors promotes migraine co-morbidity in endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Grace J Lee,Veronica Hode,Teodora Georgieva,Jill Rau,David W Dodick,Todd J Schwedt,Volker Neugebauer,Frank Porreca,Edita Navratilova

The journal of pain 25:428-450 PubMed37777035

2023

Epigenetic HDAC5 Inhibitor Reverses Craniofacial Neuropathic Pain in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Karin N Westlund,Marena Montera,Aleyah E Goins,Mark W Shilling,Mitra Afaghpour-Becklund,Sascha R A Alles,S Elise Hui

Frontiers in cell and developmental biology 11:1134874 PubMed37009475

2023

Decidual cells and decidualization in the carnivoran endotheliochorial placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Mónica Elizabeth Diessler,Rocío Hernández,Gimena Gomez Castro,Claudio Gustavo Barbeito

Pain 164:e263-e273 PubMed36625833

2023

Kappa opioid receptor agonists produce sexually dimorphic and prolactin-dependent hyperalgesic priming.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline M Kopruszinski,Moe Watanabe,Ashley L Martinez,Luiz Henrique Moreira de Souza,David W Dodick,Aubin Moutal,Volker Neugebauer,Frank Porreca,Edita Navratilova

Brain : a journal of neurology 145:2894-2909 PubMed35325034

2022

Dysregulation of serum prolactin links the hypothalamus with female nociceptors to promote migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Moe Watanabe,Caroline M Kopruszinski,Aubin Moutal,Daigo Ikegami,Rajesh Khanna,Yanxia Chen,Sarah Ross,Kimberly Mackenzie,Jennifer Stratton,David W Dodick,Edita Navratilova,Frank Porreca

American journal of cancer research 12:396-426 PubMed35141026

2022

Novel insights linking BRCA1-IRIS role in mammary gland development to formation of aggressive PABCs: the case for longer breastfeeding.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Castillo,Omonigho Aisagbonhi,Cheryl C Saenz,Wael M ElShamy

Journal of neuroinflammation 18:303 PubMed34952606

2021

Interplay between Müller cells and microglia aggravates retinal inflammatory response in experimental glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hu,Guo-Li Zhao,Meng-Xi Xu,Han Zhou,Fang Li,Yanying Miao,Bo Lei,Xiong-Li Yang,Zhongfeng Wang

Journal of morphology 282:720-732 PubMed33638264

2021

Prenatal development in Lagostomus maximus (Rodentia, Chinchillidae): A unique case among eutherian mammals of physiological embryonic death.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Acuña,Claudio G Barbeito,Enrique L Portiansky,María A Miglino,Mirta A Flamini

Heliyon 6:e03797 PubMed32322744

2020

Effects of prolactin on ventricular myocyte shortening and calcium transport in the streptozotocin-induced diabetic rat.

Applications

Unspecified application

Species

Unspecified reactive species

Frank C Howarth,Gunnar Norstedt,Oleksiy I Boldyriev,Muhammad A Qureshi,Ozaz Mohamed,Khatija Parekh,Balaji Venkataraman,Sandeep Subramanya,Anatoliy Shmygol,Lina T Al Kury

Journal of cellular physiology 235:294-303 PubMed31206688

2019

Mst1 deletion reduces hyperglycemia-mediated vascular dysfunction via attenuating mitochondrial fission and modulating the JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Qin,Dong Lin,Lina Zhang,Fei Xiao,Lixin Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prolactin Receptor/PRL-R antibody [U5]

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Flow Cytometry - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (AB2772)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Epitope

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

Product promise