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Anti-proNGF antibody [EP1320Y] (ab52918) is a rabbit monoclonal antibody that is used to detect proNGF in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 80 publications

-Over 80 publications


Images

Western blot - Anti-proNGF antibody [EP1320Y] (AB52918), expandable thumbnail
  • Western blot - Anti-proNGF antibody [EP1320Y] (AB52918), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] (AB52918), expandable thumbnail
  • Western blot - Anti-proNGF antibody [EP1320Y] (AB52918), expandable thumbnail
  • Western blot - Anti-proNGF antibody [EP1320Y] (AB52918), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Expected
Rat
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

For unpurified, use 1/500.

Species
Rat
Dilution info
1/1000
Notes

For unpurified, use 1/500.

Species
Human
Dilution info
1/1000
Notes

For unpurified, use 1/500.

Tested
Tested

Species
Human
Dilution info
1/300
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

2 products for Alternative Version

Target data

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

Alternative names

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

Recommended products

Anti-proNGF antibody [EP1320Y] (ab52918) is a rabbit monoclonal antibody that is used to detect proNGF in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 80 publications

-Over 80 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1320Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-proNGF antibody [EP1320Y] (ab52918) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of proNGF?


Anti-proNGF [EP1320Y] (ab52918) specifically detects a band for proNGF (UniProt: P01138) at a molecular weight of 27kDa.

Recommended positive controls


WB: Human fetal brain, cerebellum, HeLa, MCF-7, and MDA-MB-231 cell lysates. Mouse brain, cerebellum, and thyroid cell lysates; Rat brain, cerebellum, and thyroid cell lysates.ICC/IF: U87-MG cells.

Trusted by the scientific community


Anti-proNGF [EP1320Y] (ab52918) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EP1320Y] also available for your convenience:
ab52918, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-proNGF antibody [EP1320Y] ab196178, Carrier free - Anti-proNGF antibody [EP1320Y] - Low endotoxin, Azide free ab221609, Carrier free - Anti-proNGF antibody [EP1320Y] - BSA and Azide free ab271847, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-NGF antibody [EP1320Y] ab311770, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-NGF antibody [EP1320Y] ab313051, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-proNGF antibody [EP1320Y] ab321672



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein known as proNGF also referred to as precursor nerve growth factor is the biological precursor of mature NGF. ProNGF is significantly larger than NGF with a molecular weight of approximately 35 kDa. It is mainly expressed in the nervous system including the brain and peripheral tissues. The synthesis of proNGF occurs in various types of neurons and glial cells and it serves as a precursor that undergoes proteolytic cleavage to become the biologically active form NGF.

Biological function summary

ProNGF plays a role in modulating neuronal survival growth and differentiation. As a part of a larger signaling complex it interacts with receptors such as p75NTR and sortilin which influence the fate of neurons. Unlike its mature form proNGF tends to be associated with inducing apoptosis under certain conditions. The balance between proNGF and NGF levels in tissues can affect neural health and function significantly.

Pathways

The interactions of proNGF impact key neurological pathways. It has been implicated in the JNK signaling pathway which is important for stress response and apoptosis. ProNGF also relates to the PI3K/Akt pathway due to its interaction partners influencing cell survival and growth. Receptor binding with proteins such as TrkA and p75NTR directly affects downstream signaling cascades integrating proNGF into broader neurotrophic signaling networks.

Associated diseases and disorders

Imbalances in proNGF activity relate to the progression of neurodegenerative conditions like Alzheimer's disease where it may contribute to abnormal apoptosis in the brain. Studies also suggest its involvement in peripheral neuropathies where changes in proNGF levels lead to altered nerve function. ProNGF along with related proteins like sortilin and p75NTR could serve as potential biomarkers or therapeutic targets in managing such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-proNGF antibody [EP1320Y] (ab52918), expandable thumbnail

    Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)

    The positive cell lines (Lanes 7-9) are consistent with the literature: PMID:12944907, Fig1.

    Blocking buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Rat brain lysate at 20 µg

    Lane 3: Human cerebellum lysate at 20 µg

    Lane 4: Mouse cerebellum lysate at 20 µg

    Lane 5: Rat cerebellum lysate at 20 µg

    Lane 6: Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 7: MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 8: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa

    Observed band size: 32 kDa

    Exposure time: 20s

  • Western blot - Anti-proNGF antibody [EP1320Y] (ab52918), expandable thumbnail

    Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: Human fetal thymus lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 27 kDa

    Observed band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] (ab52918), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] (ab52918)

    Immunofluorescence staining of U87-MG cells with purified ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) . The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Western blot - Anti-proNGF antibody [EP1320Y] (ab52918), expandable thumbnail

    Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-proNGF antibody [EP1320Y] (ab52918) at 1/1000 dilution

    All lanes: Mouse thyroid lysate at 20 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 27 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-proNGF antibody [EP1320Y] (ab52918), expandable thumbnail

    Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-proNGF antibody [EP1320Y] (ab52918) at 1/2000 dilution

    All lanes: Rat thyroid lysate at 20 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 27 kDa

    Observed band size: 32 kDa

  • Western blot - Anti-proNGF antibody [EP1320Y] (ab52918), expandable thumbnail

    Western blot - Anti-proNGF antibody [EP1320Y] (ab52918)

    All lanes: Western blot - Anti-proNGF antibody [EP1320Y] (ab52918) at 1/500 dilution

    All lanes: fetal thyroid tissue at 10 µg

    Secondary

    All lanes: Goat anti rabbit IgG HRP antibody at 1/2000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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