Rabbit Recombinant Monoclonal NGF antibody. Propeptide domain. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (AB271847), expandable thumbnail](https://content.abcam.com/products/images/prongf-antibody-ep1320y-bsa-and-azide-free-ab271847--immunocytochemistry-immunofluorescence-img125837.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (AB271847)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For unpurified, use 1/500. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
Alternative names
NGFB, NGF, Beta-nerve growth factor, Beta-NGF
Recommended products
Rabbit Recombinant Monoclonal NGF antibody. Propeptide domain. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1320Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab271847 is the carrier-free version of Anti-proNGF antibody [EP1320Y] ab52918.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as proNGF also referred to as precursor nerve growth factor is the biological precursor of mature NGF. ProNGF is significantly larger than NGF with a molecular weight of approximately 35 kDa. It is mainly expressed in the nervous system including the brain and peripheral tissues. The synthesis of proNGF occurs in various types of neurons and glial cells and it serves as a precursor that undergoes proteolytic cleavage to become the biologically active form NGF.
- Biological function summary
ProNGF plays a role in modulating neuronal survival growth and differentiation. As a part of a larger signaling complex it interacts with receptors such as p75NTR and sortilin which influence the fate of neurons. Unlike its mature form proNGF tends to be associated with inducing apoptosis under certain conditions. The balance between proNGF and NGF levels in tissues can affect neural health and function significantly.
- Pathways
The interactions of proNGF impact key neurological pathways. It has been implicated in the JNK signaling pathway which is important for stress response and apoptosis. ProNGF also relates to the PI3K/Akt pathway due to its interaction partners influencing cell survival and growth. Receptor binding with proteins such as TrkA and p75NTR directly affects downstream signaling cascades integrating proNGF into broader neurotrophic signaling networks.
- Associated diseases and disorders
Imbalances in proNGF activity relate to the progression of neurodegenerative conditions like Alzheimer's disease where it may contribute to abnormal apoptosis in the brain. Studies also suggest its involvement in peripheral neuropathies where changes in proNGF levels lead to altered nerve function. ProNGF along with related proteins like sortilin and p75NTR could serve as potential biomarkers or therapeutic targets in managing such conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847), expandable thumbnail](https://content.abcam.com/products/images/prongf-antibody-ep1320y-bsa-and-azide-free-ab271847--immunocytochemistry-immunofluorescence-img125837.jpg "Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)")
Immunocytochemistry/ Immunofluorescence - Anti-proNGF antibody [EP1320Y] - BSA and Azide free (ab271847)
Immunofluorescence staining of U87-MG cells with purified Anti-proNGF antibody [EP1320Y] ab52918 at a working dilution of 1 in 300, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) . The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified Anti-proNGF antibody [EP1320Y] ab52918 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (ab15007) were used.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-proNGF antibody [EP1320Y] ab52918).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com