AB254275

Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free

Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal Properdin/PFC antibody. Carrier free. Suitable for ELISA, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

PFC, CFP, Properdin, Complement factor P

3 Images

Flow Cytometry (Intracellular) - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) treated with UV-C 50 J/m2, then recovery in serum-free medium for 30h (Right) / Untreated control (Left) labelling Properdin/PFC with ab252531 at 1/50 dilution (1μg). Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

ELISA

Supplier Data

ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

ELISA - Anti-Properdin/PFC antibody [3A3E1] (ab252531).

Antigen : Human Properdin 1000 ng/ml.

ab252531 used at 0-5000 ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).

Supplier Data

Western blot - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24797388).

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).

All lanes:

Western blot - Anti-Properdin/PFC antibody [3A3E1] (<a href='/en-us/products/primary-antibodies/properdin-pfc-antibody-3a3e1-ab252531'>ab252531</a>) at 0.454 µg/mL

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human serum lysate at 20 µg

Lane 3:

Mouse plasma lysate at 20 µg

Lane 4:

Mouse serum lysate at 20 µg

Lane 5:

Rat plasma lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 55 kDa

false

Exposure time: 1s

Unconjugated

Anti-Properdin/PFC antibody [3A3E1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A3E1

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ELISA, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "1250 - 5000 ng/ml.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.454 µg/mL", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.454 µg/mL", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.454 µg/mL", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab254275 is the carrier-free version of ab252531.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Properdin also known as factor P or complement factor P plays an essential role in the immune system. It is a serum protein with a molecular mass of approximately 53 kDa. Properdin is most commonly expressed in monocytes neutrophils and activated macrophages. It serves as a positive regulator in the alternative pathway of the complement system providing stability to the complement convertase complexes C3bBb and C3bBbC3b.

Biological function summary

Properdin enhances the activity of the complement system by stabilizing the C3 and C5 convertase enzyme complexes. These complexes are critical in the amplification of the complement cascade leading to pathogen opsonization and lysis. Properdin does not form part of a complex alone but helps existing complement complexes carry out their functions more effectively. By supporting these complexes properdin contributes to immune surveillance and the host's response to microbial invaders.

Pathways

Properdin operates within the alternative complement pathway and plays a pivotal role in immune defense. It interacts with other components such as C3 and C5 contributing to the pathway's ability to clear pathogens and damaged cells. Properdin's activity also indirectly influences other related proteins in the complement system supporting a coordinated immune response that involves factors beyond the alternative pathway itself.

Properdin deficiencies or dysregulations can lead to increased susceptibility to infections particularly by Neisseria species accounting for recurrent meningococcal disease. Additionally abnormal properdin function links to autoimmune conditions such as systemic lupus erythematosus. This connection is due to its relationship with various complement factors such as C3 which are vital for maintaining immune homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A positive regulator of the alternate pathway (AP) of complement (PubMed : 16301317, PubMed : 20382442, PubMed : 28264884, PubMed : 9748277). It binds to and stabilizes the C3- and C5-convertase enzyme complexes (PubMed : 16301317, PubMed : 20382442, PubMed : 28264884, PubMed : 9748277). Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b) (PubMed : 31507604).

See full target information CFP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 196:4671-4680 PubMed27183616

2016

Properdin-Mediated C5a Production Enhances Stable Binding of Platelets to Granulocytes in Human Whole Blood.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Z Blatt,Gurpanna Saggu,Koustubh V Kulkarni,Claudio Cortes,Joshua M Thurman,Daniel Ricklin,John D Lambris,Jesus G Valenzuela,Viviana P Ferreira

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

Western blot - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (AB254275)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise