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AB182141

Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]

3

(1 Review)

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(15 Publications)

Rabbit Recombinant Monoclonal Prostaglandin D Synthase (Lipocalin)/PDS antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 15 publications.

View Alternative Names

PDS, PTGDS, Prostaglandin-H2 D-isomerase, Beta-trace protein, Cerebrin-28, Glutathione-independent PGD synthase, Lipocalin-type prostaglandin-D synthase, Prostaglandin-D2 synthase, L-PGDS, PGD2 synthase, PGDS, PGDS2

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

The human glioma paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab182141 at 1/200 dilution for 30 mins at room temperature, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

HepG2 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab182141 at 1/200 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Rabbit secondary antibody (ab150081) at 1/1000 dilution at RT for 45 min. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab182141 overnight at 4° C. Nucleus were visualized using DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • WB

Supplier Data

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

All lanes:

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 28 kDa

false

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • WB

Supplier Data

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary and secondary antibodies : incubated for 1h at room temperature.

Lanes 1 - 3:

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/10000 dilution

Lanes 1 - 3:

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/prostaglandin-d-synthase-lipocalin-pds-antibody-ep12357-bsa-and-azide-free-ab236119'>ab236119</a>)

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 15 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Predicted band size: 28 kDa

true

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)
  • WB

Supplier Data

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (AB182141)

All lanes:

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 21 kDa

Observed band size: 28 kDa

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]

  • Carrier free

    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP12357

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB182784

Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody (AB182784)

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin D Synthase (Lipocalin) also known as PGDS or PDS is an enzyme that catalyzes the conversion of prostaglandin H2 to prostaglandin D2. It plays an important mechanical role in prostaglandin metabolism and has a mass of approximately 20 kDa. This protein is prominently expressed in the central nervous system heart and male genital organs. Its alternate name is lipocalin-type prostaglandin D synthase highlighting its membership in the lipocalin family. Notably it is abundant in cerebrospinal fluid and seminal plasma.
Biological function summary

This protein PDS participates in various physiological processes including inflammation and sleep regulation. It operates independently and is not typically part of larger protein complexes. PGDS contributes to the homeostasis of biological membranes and acts as a neuromodulator in the brain. Its function extends to mediating the actions of the prostaglandin D2 it produces affecting processes like allergic reactions and maintaining normal reproductive physiological functions.

Pathways

PGDS engages with key biological processes by being part of the MAPK signaling pathway and the arachidonic acid cascade. These pathways contribute to inflammation and immune response regulation. PGDS interacts specifically with other proteins along these pathways such as cyclooxygenases (COX-1 COX-2) which are important in the initial creation of prostaglandin H2. Its role in signaling elucidates its involvement in both central and peripheral biological activities.

PGDS links to conditions such as sleep disorders and allergic diseases. The production of prostaglandin D2 by PGDS can exacerbate symptoms of certain allergic responses including asthma. Furthermore PGDS involvement in sleep regulation can relate to narcolepsy. In these conditions it interacts with other proteins involved in prostaglandin signaling such as COX enzymes which are targeted by certain therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation (PubMed : 20667974). Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system (PubMed : 20667974, PubMed : 9475419). Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR (By similarity).
See full target information PTGDS
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:31 PubMed40408096

2025

Single-Cell RNA Sequencing of the Primary Visual Cortex in Mice With Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Deling Li,Bin Zou,Qinyuan Hu,Xinyi Zhang,Weiting Zeng,Liling Liu,Minbin Yu

British journal of cancer 132:384-400 PubMed39706989

2024

Targeting PTGDS Promotes ferroptosis in peripheral T cell lymphoma through regulating HMOX1-mediated iron metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shunfeng Hu,Bingyu Liu,Juanjuan Shang,Qianqian Guo,Tiange Lu,Xiaoli Zhou,Xiangxiang Zhou,Xin Wang

Cells 12: PubMed36831222

2023

CXXC5 Mediates DHT-Induced Androgenetic Alopecia via PGD.

Applications

Unspecified application

Species

Unspecified reactive species

Yeong Chan Ryu,Jiyeon Park,You-Rin Kim,Sehee Choi,Geon-Uk Kim,Eunhwan Kim,Yumi Hwang,Heejene Kim,Gyoonhee Han,Soung-Hoon Lee,Kang-Yell Choi

Cell death discovery 9:39 PubMed36725845

2023

PGD2 displays distinct effects in diffuse large B-cell lymphoma depending on different concentrations.

Applications

Unspecified application

Species

Unspecified reactive species

Shunfeng Hu,Tiange Lu,Juanjuan Shang,Yiqing Cai,Mengfei Ding,Xiangxiang Zhou,Xin Wang

Cell death and differentiation 29:642-656 PubMed34743203

2021

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shunfeng Hu,Shuai Ren,Yiqing Cai,Jiarui Liu,Yang Han,Yi Zhao,Juan Yang,Xiangxiang Zhou,Xin Wang

Nature communications 12:2506 PubMed33947851

2021

Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Diao,Chenhui Wang,Rongshuai Wang,Zeqing Feng,Ji Zhang,Han Yang,Yingjun Tan,Huiming Wang,Changsong Wang,Liang Liu,Ying Liu,Yueping Liu,Gang Wang,Zilin Yuan,Xiaotao Hou,Liang Ren,Yuzhang Wu,Yongwen Chen

Biological psychiatry 91:129-140 PubMed33309016

2020

Sex Differences in Nociceptor Translatomes Contribute to Divergent Prostaglandin Signaling in Male and Female Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Tavares-Ferreira,Pradipta R Ray,Ishwarya Sankaranarayanan,Galo L Mejia,Andi Wangzhou,Stephanie Shiers,Ruta Uttarkar,Salim Megat,Paulino Barragan-Iglesias,Gregory Dussor,Armen N Akopian,Theodore J Price

Scientific reports 10:13108 PubMed32753632

2020

The prostaglandin synthases, COX-2 and L-PGDS, mediate prostate hyperplasia induced by low-dose bisphenol A.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangshuang Wu,Dongyan Huang,Xin Su,Han Yan,Aicui Ma,Lei Li,Jianhui Wu,Zuyue Sun

Pain practice : the official journal of World Institute of Pain 21:64-74 PubMed32640501

2020

Dexamethasone Suppresses Radicular Pain Through Targeting the L-PGDS/PI3K/Akt Pathway in Rats With Lumbar Disc Herniation.

Applications

Unspecified application

Species

Unspecified reactive species

Weixing Xu,Weiguo Ding,Hongfeng Sheng,Di Lu,Xinwei Xu,Bin Xu

Aging 11:9500-9529 PubMed31708494

2019

ER-depletion lowering the 'hypothalamus-uterus-kidney' axis functions by perturbing the renal ERβ/Ptgds signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ru Liu,Zhi-Shu Tang,Jin-Ao Duan,Lin Chen,Jing Sun,Rui Zhou,Zhong-Xing Song,Xin-Bo Shi,Hui-Yuan Zhu
View all publications
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Product promise

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