Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (AB236119)

The human glioma paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab182141 at 1/200 dilution for 30 mins at room temperature, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (AB236119)

HepG2 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab182141 at 1/200 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Rabbit secondary antibody (ab150081) at 1/1000 dilution at RT for 45 min. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab182141 overnight at 4° C. Nucleus were visualized using DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (AB236119)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (AB236119)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (AB236119)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Primary and secondary antibodies : incubated for 1h at room temperature. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

Lanes 1 - 3:

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (<a href='/en-us/products/primary-antibodies/prostaglandin-d-synthase-lipocalin-pds-antibody-ep12357-ab182141'>ab182141</a>) at 1/10000 dilution

Lanes 1 - 3:

Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (ab236119)

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 15 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Predicted band size: 28 kDa

true