AB168621

Anti-Prostaglandin E Synthase/MPGES-1 antibody

Name: Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab168621) | Abcam
Brand: Abcam Limited
SKU: AB168621

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(2 Publications)

Mouse Polyclonal Prostaglandin E Synthase/MPGES-1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PTGES.

View Alternative Names

MGST1L1, MPGES1, PGES, PIG12, PTGES, Prostaglandin E synthase, Glutathione peroxidase PTGES, Glutathione transferase PTGES, Microsomal glutathione S-transferase 1-like 1, Microsomal prostaglandin E synthase 1, p53-induced gene 12 protein, MGST1-L1, MPGES-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB168621)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB168621)

Immunofluorescent analysis of HeLa cells labeling Prostaglandin E Synthase/MPGES-1 with ab168621 at 10 μg/ml.

Unknown

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB168621)

All lanes:

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab168621) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 17 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PTGES.

O14684

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Synthase (MPGES-1) also known as prostin E is a membrane-associated protein that catalyzes the conversion of prostaglandin H2 to prostaglandin E2. It has a molecular mass of about 16 kDa. MPGES-1 is widely expressed in various tissues including the kidneys lungs and heart. The expression of MPGES-1 increases in response to pro-inflammatory signals highlighting its adaptive response during inflammation.

Biological function summary

Prostaglandin E Synthase plays an important role in mediating inflammatory responses and is part of the terminal enzyme complex in the prostaglandin synthesis pathway. Its activity contributes to the elevated production of prostaglandin E2 (PGE2) a bioactive lipid that regulates inflammation fever and pain. MPGES-1 functions in concert with Cyclooxygenase-2 (COX-2) as part of the coordinated induction during inflammatory stimuli.

Pathways

The MPGES-1 enzyme is central to the arachidonic acid cascade specifically in the cyclooxygenase pathway. This pathway leads to the production of prostaglandins which are involved in inflammatory and immune responses. Prostaglandin E2 produced by MPGES-1 is related to proteins such as COX-2 and cytosolic phospholipase A2 (cPLA2) which precede it in the biosynthesis pathway and act collaboratively in the metabolism of arachidonic acid to prostanoids.

MPGES-1 is implicated particularly in conditions like rheumatoid arthritis and cancer. Elevated levels of prostaglandin E2 produced by the concerted action of MPGES-1 have been observed in inflamed joints of arthritis patients correlating with COX-2 expression. In cancer upregulation of MPGES-1 contributes to tumorigenesis through promoting angiogenesis and proliferation. Its interaction with COX-2 enhances oncogenic processes signifying its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Terminal enzyme of the cyclooxygenase (COX)-2-mediated prostaglandin E2 (PGE2) biosynthetic pathway. Catalyzes the glutathione-dependent oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) in response to inflammatory stimuli (PubMed : 10377395, PubMed : 10869354, PubMed : 12244105, PubMed : 12460774, PubMed : 12672824, PubMed : 18682561). Plays a key role in inflammation response, fever and pain (By similarity). Also catalyzes the oxidoreduction of endocannabinoids into prostaglandin glycerol esters and PGG2 into 15-hydroperoxy-PGE2 (PubMed : 12244105, PubMed : 12672824). In addition, displays low glutathione transferase and glutathione-dependent peroxidase activities, toward 1-chloro-2,4-dinitrobenzene and 5-hydroperoxyicosatetraenoic acid (5-HPETE), respectively (PubMed : 12672824).

See full target information PTGES

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed38139073

2023

Protects against Primary Dysmenorrhea in Mice by Inhibiting Inflammatory Response and Regulating the COX2/PGE2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Yang,Yunyuan Tian,Jincai Liu,Yaoyao Kou,Yanhua Xie,Siwang Wang,Ye Zhao

Molecular medicine reports 16:9347-9354 PubMed29039590

2017

MicroRNA‑218 promotes prostaglandin E2 to inhibit osteogenic differentiation in synovial mesenchymal stem cells by targeting 15‑hydroxyprostaglandin dehydrogenase [NAD(+)].

Applications

Unspecified application

Species

Unspecified reactive species

Ruijun Cong,Kun Tao,Peiliang Fu,Lieming Lou,Yuchang Zhu,Song Chen,Xinyu Cai,Lingzhou Mao

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Prostaglandin E Synthase/MPGES-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB168621)

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB168621)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise