AB62050

Anti-Prostaglandin E Synthase/MPGES-1 antibody

Name: Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050) | Abcam
Brand: Abcam Limited
SKU: AB62050
Rating: 5 (3 reviews)

(3 Reviews)

(16 Publications)

Rabbit Polyclonal Prostaglandin E Synthase/MPGES-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 16 publications.

View Alternative Names

MGST1L1, MPGES1, PGES, PIG12, PTGES, Prostaglandin E synthase, Glutathione peroxidase PTGES, Glutathione transferase PTGES, Microsomal glutathione S-transferase 1-like 1, Microsomal prostaglandin E synthase 1, p53-induced gene 12 protein, MGST1-L1, MPGES-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB62050)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB62050)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, staining Prostaglandin E Synthase/MPGES-1 with ab62050 at 10 μg/ml.

IHC-P

AbReview39564****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB62050)

ab62050 staining Prostaglandin E Synthase/MPGES-1 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton-X and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 20 hours at 4°C. ab150075, a donkey anti-rabbit Alexa Fluor® 647 (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Synthase (MPGES-1) also known as prostin E is a membrane-associated protein that catalyzes the conversion of prostaglandin H2 to prostaglandin E2. It has a molecular mass of about 16 kDa. MPGES-1 is widely expressed in various tissues including the kidneys lungs and heart. The expression of MPGES-1 increases in response to pro-inflammatory signals highlighting its adaptive response during inflammation.

Biological function summary

Prostaglandin E Synthase plays an important role in mediating inflammatory responses and is part of the terminal enzyme complex in the prostaglandin synthesis pathway. Its activity contributes to the elevated production of prostaglandin E2 (PGE2) a bioactive lipid that regulates inflammation fever and pain. MPGES-1 functions in concert with Cyclooxygenase-2 (COX-2) as part of the coordinated induction during inflammatory stimuli.

Pathways

The MPGES-1 enzyme is central to the arachidonic acid cascade specifically in the cyclooxygenase pathway. This pathway leads to the production of prostaglandins which are involved in inflammatory and immune responses. Prostaglandin E2 produced by MPGES-1 is related to proteins such as COX-2 and cytosolic phospholipase A2 (cPLA2) which precede it in the biosynthesis pathway and act collaboratively in the metabolism of arachidonic acid to prostanoids.

MPGES-1 is implicated particularly in conditions like rheumatoid arthritis and cancer. Elevated levels of prostaglandin E2 produced by the concerted action of MPGES-1 have been observed in inflamed joints of arthritis patients correlating with COX-2 expression. In cancer upregulation of MPGES-1 contributes to tumorigenesis through promoting angiogenesis and proliferation. Its interaction with COX-2 enhances oncogenic processes signifying its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Terminal enzyme of the cyclooxygenase (COX)-2-mediated prostaglandin E2 (PGE2) biosynthetic pathway. Catalyzes the glutathione-dependent oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) in response to inflammatory stimuli (PubMed : 10377395, PubMed : 10869354, PubMed : 12244105, PubMed : 12460774, PubMed : 12672824, PubMed : 18682561). Plays a key role in inflammation response, fever and pain (By similarity). Also catalyzes the oxidoreduction of endocannabinoids into prostaglandin glycerol esters and PGG2 into 15-hydroperoxy-PGE2 (PubMed : 12244105, PubMed : 12672824). In addition, displays low glutathione transferase and glutathione-dependent peroxidase activities, toward 1-chloro-2,4-dinitrobenzene and 5-hydroperoxyicosatetraenoic acid (5-HPETE), respectively (PubMed : 12672824).

See full target information PTGES

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Parasitology research 124:9 PubMed39832004

2025

Activation of the COX-2/mPGES-1/PGE-2 cascade through the NLRP3 inflammasome contributes to Angiostrongylus cantonensis-induced eosinophilic meningoencephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Min Chen,Cheng-You Lu,Shih-Chan Lai

Journal of the American Heart Association 14:e036762 PubMed39817553

2025

Janus Kinase 2/Signal Transducer and Activator of Transcription 3/Cyclooxygenase 2 Signaling Pathway Mediates the Effect of Central Angiotensin II on the Elevation of Rostral Ventrolateral Medulla Prostaglandin E-Induced Oxidative Stress in Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Fu Fan,Xing Tan,Wen Wang,Ji-Kui Li,Yu-Chen Xiao,Wei-Zhong Wang,Yang-Kai Wang

Antioxidants (Basel, Switzerland) 12: PubMed37372034

2023

Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rocío Muñoz-García,Marina Sánchez-Hidalgo,Manuel Alcarranza,María Victoria Vazquéz-Román,María Alvarez de Sotomayor,María Luisa González-Rodríguez,María C de Andrés,Catalina Alarcón-de-la-Lastra

Anatomia, histologia, embryologia 51:289-299 PubMed35132701

2022

Regulatory changes of local produced prostaglandins in corpus luteum after experimentally induced luteolysis in the cow.

Applications

Unspecified application

Species

Unspecified reactive species

Bajram Berisha,Granit Thaqi,Daniela Rodler,Dieter Schams,Fred Sinowatz,Michael W Pfaffl

Aging 13:14304-14321 PubMed34016791

2021

Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wu,Xinyi Zheng,Zhixian Yao,Zhong Zheng,Wenjie Huang,Xingyu Mu,Feng Sun,Zhihong Liu,Junhua Zheng

Journal for immunotherapy of cancer 9: PubMed33495298

2021

MUC1-C integrates activation of the IFN-γ pathway with suppression of the tumor immune microenvironment in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nami Yamashita,Mark Long,Atsushi Fushimi,Masaaki Yamamoto,Tsuyoshi Hata,Masayuki Hagiwara,Atrayee Bhattacharya,Qiang Hu,Kwok-Kin Wong,Song Liu,Donald Kufe

Nature 590:122-128 PubMed33473210

2021

Restoring metabolism of myeloid cells reverses cognitive decline in ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Paras S Minhas,Amira Latif-Hernandez,Melanie R McReynolds,Aarooran S Durairaj,Qian Wang,Amanda Rubin,Amit U Joshi,Joy Q He,Esha Gauba,Ling Liu,Congcong Wang,Miles Linde,Yuki Sugiura,Peter K Moon,Ravi Majeti,Makoto Suematsu,Daria Mochly-Rosen,Irving L Weissman,Frank M Longo,Joshua D Rabinowitz,Katrin I Andreasson

Biochemical and biophysical research communication 527:42-48 PubMed32446389

2020

In vitro and in vivo impairment of embryo implantation by commonly used fungicide Mancozeb.

Applications

Unspecified application

Species

Unspecified reactive species

Ihshan Akthar,Ziyi Wang,Missaka P B Wijayagunawardane,Chathura J Ratnayake,Eranga H Siriweera,Kai-Fai Lee,Suranga P Kodithuwakku

OncoTargets and therapy 12:10917-10930 PubMed31849495

2019

Triterpenoid Saponins from Suppress Tumor Cell Proliferation by Regulating MAPK, PD1/PDL1, and STAT3 Signaling Pathways and Altering Cancer Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Lintao Han,Shiqi Yao,Sa Cao,Guoyan Mo,Jingjing Li,Yan Cao,Fang Huang

Frontiers in endocrinology 10:467 PubMed31354631

2019

Prostaglandins in Superovulation Induced Bovine Follicles During the Preovulatory Period and Early Corpus Luteum.

Applications

Unspecified application

Species

Unspecified reactive species

Bajram Berisha,Daniela Rodler,Dieter Schams,Fred Sinowatz,Michael W Pfaffl

View all publications

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Product promise

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Anti-Prostaglandin E Synthase/MPGES-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB62050)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (AB62050)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise