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AB250236

Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] - BSA and Azide free

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Rabbit Recombinant Monoclonal Prostaglandin E Synthase/MPGES-1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

View Alternative Names

MGST1L1, MPGES1, PGES, PIG12, PTGES, Prostaglandin E synthase, Glutathione peroxidase PTGES, Glutathione transferase PTGES, Microsomal glutathione S-transferase 1-like 1, Microsomal prostaglandin E synthase 1, p53-induced gene 12 protein, MGST1-L1, MPGES-1

2 Images
Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] - BSA and Azide free (AB250236)
  • WB

Supplier Data

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] - BSA and Azide free (AB250236)

This data was developed using ab180589, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (<a href='/en-us/products/primary-antibodies/prostaglandin-e-synthase-mpges-1-antibody-epr13765-ab180589'>ab180589</a>) at 1/1000 dilution

Lane 1:

NIH 3T3 cell line lysate at 20 µg

Lane 2:

Jurkat cell line lysate at 20 µg

Lane 3:

HeLa cell line lysate at 20 µg

Lane 4:

A549 cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 13 kDa

false

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] - BSA and Azide free (AB250236)
  • WB

Supplier Data

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] - BSA and Azide free (AB250236)

This data was developed using ab180589, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (<a href='/en-us/products/primary-antibodies/prostaglandin-e-synthase-mpges-1-antibody-epr13765-ab180589'>ab180589</a>) at 1/1000 dilution

Lane 1:

Lysate of NIH 3T3 cells at 10 µg with Immunizing peptide

Lane 2:

Lysate of NIH 3T3 cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 13 kDa

false

  • Unconjugated

    Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13765

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250236 is the carrier-free version of ab180589.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E Synthase (MPGES-1) also known as prostin E is a membrane-associated protein that catalyzes the conversion of prostaglandin H2 to prostaglandin E2. It has a molecular mass of about 16 kDa. MPGES-1 is widely expressed in various tissues including the kidneys lungs and heart. The expression of MPGES-1 increases in response to pro-inflammatory signals highlighting its adaptive response during inflammation.
Biological function summary

Prostaglandin E Synthase plays an important role in mediating inflammatory responses and is part of the terminal enzyme complex in the prostaglandin synthesis pathway. Its activity contributes to the elevated production of prostaglandin E2 (PGE2) a bioactive lipid that regulates inflammation fever and pain. MPGES-1 functions in concert with Cyclooxygenase-2 (COX-2) as part of the coordinated induction during inflammatory stimuli.

Pathways

The MPGES-1 enzyme is central to the arachidonic acid cascade specifically in the cyclooxygenase pathway. This pathway leads to the production of prostaglandins which are involved in inflammatory and immune responses. Prostaglandin E2 produced by MPGES-1 is related to proteins such as COX-2 and cytosolic phospholipase A2 (cPLA2) which precede it in the biosynthesis pathway and act collaboratively in the metabolism of arachidonic acid to prostanoids.

MPGES-1 is implicated particularly in conditions like rheumatoid arthritis and cancer. Elevated levels of prostaglandin E2 produced by the concerted action of MPGES-1 have been observed in inflamed joints of arthritis patients correlating with COX-2 expression. In cancer upregulation of MPGES-1 contributes to tumorigenesis through promoting angiogenesis and proliferation. Its interaction with COX-2 enhances oncogenic processes signifying its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Terminal enzyme of the cyclooxygenase (COX)-2-mediated prostaglandin E2 (PGE2) biosynthetic pathway. Catalyzes the glutathione-dependent oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) in response to inflammatory stimuli (PubMed : 10377395, PubMed : 10869354, PubMed : 12244105, PubMed : 12460774, PubMed : 12672824, PubMed : 18682561). Plays a key role in inflammation response, fever and pain (By similarity). Catalyzes also the oxidoreduction of endocannabinoids into prostaglandin glycerol esters and PGG2 into 15-hydroperoxy-PGE2 (PubMed : 12244105, PubMed : 12672824). In addition, displays low glutathione transferase and glutathione-dependent peroxidase activities, toward 1-chloro-2,4-dinitrobenzene and 5-hydroperoxyicosatetraenoic acid (5-HPETE), respectively (PubMed : 12672824).
See full target information PTGES
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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