AB126709

Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal Prostaglandin F2 alpha Receptor/PTGFR antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Prostaglandin F2-alpha receptor, PGF receptor, PGF2-alpha receptor, Prostanoid FP receptor, PTGFR

2 Images

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Western blot - Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844] (AB126709)

All lanes:

Western blot - Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844] (ab126709) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

T47D cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa

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OI-RD Scanning - Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844] (AB126709)

OI-RD Scanning

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OI-RD Scanning - Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844] (AB126709)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5844

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Prostaglandin F2 alpha Receptor also known as PTGFR is a G-protein-coupled receptor with a molecular mass of about 66 kDa. This receptor plays an essential role in mediating the effects of prostaglandin F2 alpha (PGF2α) a lipid compound involved in various physiological processes. The receptor is mainly expressed in tissues such as the uterus eye and vascular smooth muscle. Its localization allows it to actively participate in reproductive functions and other biological activities in these tissues.

Biological function summary

Prostaglandin F2 alpha Receptor functions as part of the prostanoid receptor family which mediates diverse physiological responses. PGF2α binding to the PTGFR induces intracellular signaling cascades that lead to smooth muscle contraction and modulation of inflammation. The receptor is not known to be part of any higher-order complex but interacts with intracellular signaling molecules to exert its effects. Its activity is important in processes such as luteolysis and modulation of intraocular pressure.

Pathways

The Prostaglandin F2 alpha Receptor is involved in key regulatory pathways like the prostaglandin signaling pathway and the smooth muscle contraction pathway. Within the prostaglandin signaling pathway PTGFR works with proteins such as phospholipase C to increase intracellular calcium levels leading to smooth muscle contraction. Its role in these pathways highlights its function in reproductive cycles and maintenance of cardiovascular health.

The Prostaglandin F2 alpha Receptor has associations with conditions such as glaucoma and dysmenorrhea. In glaucoma the receptor's role in modulating intraocular pressure links it to elevated pressure and optic nerve damage. Similarly dysmenorrhea involves excessive uterine muscle contractions where PTGFR-mediated signaling becomes heightened. The receptor's interaction with other proteins like cyclooxygenase-2 (COX-2) exacerbates these conditions by influencing prostaglandin production and signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for prostaglandin F2-alpha (PGF2-alpha). The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Initiates luteolysis in the corpus luteum (By similarity). Isoforms 2 to 7 do not bind PGF2-alpha but are proposed to modulate signaling by participating in variant receptor complexes; heterodimers between isoform 1 and isoform 5 are proposed to be a receptor for prostamides including the synthetic analog bimatoprost.

See full target information Prostaglandin F2-alpha receptor

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 22:278-282 PubMed39658593

2024

A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Kejia Li,Shijie Chen,Keyun Wang,Yan Wang,Lianji Xue,Yuying Ye,Zheng Fang,Jiawen Lyu,Haiyang Zhu,Yanan Li,Ting Yu,Feng Yang,Xiaolei Zhang,Siqi Guo,Chengfei Ruan,Jiahua Zhou,Qi Wang,Mingming Dong,Cheng Luo,Mingliang Ye

Frontiers in genetics 13:902329 PubMed35938015

2022

Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chengmao Xie,Ziran Yin,Yong Liu

Frontiers in endocrinology 12:652807 PubMed33868179

2021

The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Meihe Li,Yang Haixia,Minchao Kang,Peng An,Xili Wu,Huimin Dang,Xin Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844]

Western blot - Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844] (AB126709)

OI-RD Scanning - Anti-Prostaglandin F2 alpha Receptor/PTGFR antibody [EPR5844] (AB126709)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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