AB403

Anti-Prostate Specific Antigen antibody [8301]

Name: Anti-Prostate Specific Antigen antibody [8301] (ab403) | Abcam
Brand: Abcam Limited
SKU: AB403
Price: 435 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(9 Publications )

Anti-Prostate Specific Antigen antibody [8301] (ab403) is a mouse monoclonal antibody detecting Prostate Specific Antigen in IHC-P. Suitable for Human.

- Trusted since 1999

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (AB403)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (AB403)

Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control. Sections were stained using an automated system, at room temperature : sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8301

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human KLK3.

P07288

Specificity

Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59%Kallikrein - <0.04% PAP - <0.04% Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays.Affinity constant: 1x10<sup>11</sup> l/mol human prostate-specific antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Prostate Specific Antigen antibody [8301] (ab403) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 6 Preservative: 0.095% Sodium azide Constituents: PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.

Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

See full target information KLK3

Alternative Names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

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Publications (9)

Recent publications for all applications. Explore the full listand refine your search

Nanoscale 17:11401-11412 PubMed40231544

2025

Bioconjugates of photon-upconversion nanoparticles with antibodies for the detection of prostate-specific antigen and p53 in heterogeneous and homogeneous immunoassays.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina Makhneva,Pavel Špaček,Antonín Hlaváček,Julie Weisová,Hans H Gorris,Petr Skládal,Zdeněk Farka

Communications engineering 2: PubMed38586601

2023

Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen Brosamer,Katerina Kourentzi,Richard C Willson,Binh V Vu

Nature protocols 17:1028-1072 PubMed35181766

2022

Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Antonín Hlaváček,Zdeněk Farka,Matthias J Mickert,Uliana Kostiv,Julian C Brandmeier,Daniel Horák,Petr Skládal,František Foret,Hans H Gorris

The Prostate 80:1145-1156 PubMed32659025

2020

CXCL12γ induces human prostate and mammary gland development.

Applications

Unspecified application

Species

Unspecified reactive species

Younghun Jung,Jin Koo Kim,Eunsohl Lee,Frank C Cackowski,Ann M Decker,Paul H Krebsbach,Russell S Taichman

Analytical methods : advancing methods and applications 12:272-280 PubMed32577135

2020

A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors.

Applications

Unspecified application

Species

Unspecified reactive species

Adheesha N Danthanarayana,Erin Finley,Binh Vu,Katerina Kourentzi,Richard C Willson,Jakoah Brgoch

The Journal of biological chemistry 285:33045-53 PubMed20713353

2010

Phosphorylation and activation of androgen receptor by Aurora-A.

Applications

Species

Human

Shao-Kun Shu,Qiyuan Liu,Domenico Coppola,Jin Q Cheng

Analytical chemistry 81:9183-7 PubMed19874062

2009

Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes.

Applications

Unspecified application

Species

Unspecified reactive species

Dongwoo Kim,Weston L Daniel,Chad A Mirkin

Proceedings of the National Academy of Sciences of 106:18437-42 PubMed19841273

2009

Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy.

Applications

Unspecified application

Species

Human

C Shad Thaxton,Robert Elghanian,Audrey D Thomas,Savka I Stoeva,Jae-Seung Lee,Norm D Smith,Anthony J Schaeffer,Helmut Klocker,Wolfgang Horninger,Georg Bartsch,Chad A Mirkin

Nature biotechnology 23:1294-301 PubMed16170313

2005

Multiplexed electrical detection of cancer markers with nanowire sensor arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Gengfeng Zheng,Fernando Patolsky,Yi Cui,Wayne U Wang,Charles M Lieber

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prostate Specific Antigen antibody [8301]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (AB403)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (9)

Product promise