AB2218

Anti-Prostate Specific Antigen antibody [A67-B/E3]

Name: Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218) | Abcam
Brand: Abcam Limited
SKU: AB2218
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Prostate Specific Antigen antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] (AB2218)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] (AB2218)

Immunohistochemical analysis of human prostate tissue sections staining Prostate Specific Antigen with ab2218.

Unknown

Western blot - Anti-Prostate Specific Antigen antibody [A67-B/E3] (AB2218)

All lanes:

Western blot - Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218) at 5 µg/mL

All lanes:

Human Seminal Plasma

Secondary

All lanes:

IRDye800-conjugated Anti-Mouse IgG1 Secondary Antibody

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Biotin

Biotin Anti-Prostate Specific Antigen antibody [A67-B/E3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A67-B/E3

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Specificity

This antibody is specific for PSA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.

Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

See full target information KLK3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncoimmunology 11:2073050 PubMed35558161

2022

and mutations are associated with distinct blast immunophenotype in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kateřina Kuželová,Barbora Brodská,Jana Marková,Martina Petráčková,Johannes Schetelig,Šárka Ransdorfová,Zdenka Gašová,Cyril Šálek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prostate Specific Antigen antibody [A67-B/E3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] (AB2218)

Western blot - Anti-Prostate Specific Antigen antibody [A67-B/E3] (AB2218)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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