AB212480

Anti-Prostate Specific Antigen antibody [A67-B/E3] - BSA and Azide free

Name: Anti-Prostate Specific Antigen antibody [A67-B/E3] - BSA and Azide free (ab212480) | Abcam
Brand: Abcam Limited
SKU: AB212480
Price: 905 USD
Availability: InStock

(1 Review)

(1 Publication)

Mouse Monoclonal Prostate Specific Antigen antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human KLK3.

View Alternative Names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] - BSA and Azide free (AB212480)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] - BSA and Azide free (AB212480)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Prostate Specific Antigen with ab212480 at 0.5 μg/ml.

Biotin

Biotin Anti-Prostate Specific Antigen antibody [A67-B/E3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A67-B/E3

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human KLK3.

P07288

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab212480 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.

Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

See full target information KLK3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:542 PubMed36203068

2022

Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Wakao,Yo Oguma,Yoshihiro Kushida,Yasumasa Kuroda,Kazuki Tatsumi,Mari Dezawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prostate Specific Antigen antibody [A67-B/E3] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] - BSA and Azide free (AB212480)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise