Rabbit Polyclonal Prostate Specific Antigen antibody. Suitable for ELISA, WB, IHC-P and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human KLK3.
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- Scientific reports 13:166632023Pinostilbene inhibits full-length and splice variant of androgen receptor in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWon Sik Shin,Seung Hyun Han,Kyung Won Jo,Yunje Cho,Kyong-Tai KimPubMed 37794090
- Molecular metabolism 71:1017062023Inceptor correlates with markers of prostate cancer progression and modulates insulin/IGF1 signaling and cancer cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Wissmiller,Sara Bilekova,Andras Franko,Stefan Z Lutz,Miriam Katsburg,Sebastian Gulde,Natalia S Pellegata,Arnulf Stenzl,Martin Heni,Lucia Berti,Hans-Ulrich Häring,Heiko LickertPubMed 36931467
- BMC molecular and cell biology 23:512022Lncap-AI prostate cancer cell line establishment by Flutamide and androgen-free environment to promote cell adherent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuifeng Wang,Xihua Wei,Die Zhang,Weidong Li,Yanling HuPubMed 36443669
- Science advances 8:eabf90962022Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Whitburn,Srinivasa R Rao,Emma V Morris,Sho Tabata,Akiyoshi Hirayama,Tomoyoshi Soga,James R Edwards,Zeynep Kaya,Charlotte Palmer,Freddie C Hamdy,Claire M EdwardsPubMed 35213227
- Genes 11:2020Characterization of Hormone-Dependent Pathways in Six Human Prostate-Cancer Cell Lines: A Gene-Expression Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndras Franko et. al.PubMed 33036464
- Scientific reports 9:38232019Maintenance of MYC expression promotes de novo resistance to BET bromodomain inhibition in castration-resistant prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Coleman et. al.PubMed 30846826
- BMC veterinary research 14:3272018Comparison of hock- and footpad-injection as a prostate adenocarcinoma model in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenning Richter et. al.PubMed 30400793
- Pharmacology research & perspectives 6:e004372018Dual androgen receptor (AR) and STAT3 inhibition by a compound targeting the AR amino-terminal domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaping Hua et. al.PubMed 30410767
- Oncology letters 15:2218-22262018Oxibendazole inhibits prostate cancer cell growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiaoli Chen,Yuhua Li,Xiaoyu Zhou,Runsheng LiPubMed 29434928
- Oncotarget 7:40690-407032016Cellular androgen content influences enzalutamide agonism of F877L mutant androgen receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Coleman,Kathryn Van Hook,Carly J King,Jacob Schwartzman,Robert Lisac,Joshua Urrutia,Archana Sehrawat,Josha Woodward,Nicholas J Wang,Roman Gulati,George V Thomas,Tomasz M Beer,Martin Gleave,James E Korkola,Lina Gao,Laura M Heiser,Joshi J AlumkalPubMed 27276681
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KLK3. The exact immunogen used to generate this antibody is proprietary information. Database link P07288
Consider this alternative
Reactivity data
Select an application| ELISA | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Expected | Tested | Expected |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Alternative names
APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin
Recommended products
Rabbit Polyclonal Prostate Specific Antigen antibody. Suitable for ELISA, WB, IHC-P and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human KLK3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KLK3. The exact immunogen used to generate this antibody is proprietary information. Database link P07288
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
- Biological function summary
PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.
- Pathways
PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.
- Associated diseases and disorders
PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Prostate Specific Antigen antibody (ab53774)
All lanes: Western blot - Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution
Lane 1: Extracts from mouse heart
Lane 2: Extracts from mouse heart, plus immunising peptide
Predicted band size: 29 kDa
Observed band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab53774)
ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
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