Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-Prostate Specific Antigen (ab76113, red; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Nkx3.1 (ab196020, gray; Opal™570) on human prostate gland tissue. Panel B : anti-Prostate Specific Antigen stained on cytoplasm of luminal cells. Panel C : anti-Cytokeratin 5 stained on basal cells. Panel D : anti-p63 stained on nucleus of luminal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab76113 (1/2000), ab236216 (1/400) and ab196020 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope. This data was developed using ab76113, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-p63 (ab124762, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (ab76113, red; Opal™570) on human prostate gland tissue. Panel B : anti-Prostate Specific Antigen stained on luminal cells. Panel C : anti-Cytokeratin 5 stained on cytoplasm of basal cells. Panel D : anti-p63 stained on nucleus of basal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab124762 (1/5000), ab236216 (1/400), and ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope. This data was developed using ab76113, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-Collagen VI (ab271938, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (ab76113, red; Opal™570) on human prostate gland tissue. Panel B : anti-Prostate Specific Antigen stained on luminal cells. Panel C : anti-Cytokeratin 5 stained on basal cells. Panel D : anti-Collagen VI stained on stroma. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab271938 (1/500), ab236216 (1/400), and ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope. This data was developed using ab76113, the same antibody clone in a different buffer formulation.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

This data was developed using ab76113, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate cancer tissue sections labeling Prostate Specific Antigen with purified ab76113 at 1/1000 dilution (0.51 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

This data was developed using ab76113, the same antibody clone in a different buffer formulation.

The expression profile observed is consistent with what has been described in the literature (PMID : 23405127).

All lanes:

Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] (<a href='/en-us/products/primary-antibodies/prostate-specific-antigen-antibody-ep1588y-ab76113'>ab76113</a>) at 1/1000 dilution

Lane 1:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

DU 145 (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

• WB

Unknown

Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

This data was developed using ab76113, the same antibody clone in a different buffer formulation.

The expression profile observed is consistent with what has been described in the literature (PMID : 23405127).

All lanes:

Western blot - Anti-Prostate Specific Antigen antibody [EP1588Y] (<a href='/en-us/products/primary-antibodies/prostate-specific-antigen-antibody-ep1588y-ab76113'>ab76113</a>) at 1/1000 dilution

Lane 1:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

LNCaP (Human prostate carcinoma epithelial cell) treated with 100nM DHT for 2 days whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Prostate Specific Antigen antibody [EP1588Y] - BSA and Azide free (AB271858)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.