Rabbit Recombinant Monoclonal Prostate Specific Antigen antibody. Carrier free. Suitable for sELISA and reacts with Human, Rhesus monkey samples.
Images
![Sandwich ELISA - Anti-Prostate Specific Antigen antibody [EPR22271-81] - BSA and Azide free (Detector) (AB242192), expandable thumbnail](https://content.abcam.com/products/images/prostate-specific-antigen-antibody-epr22271-81-bsa-and-azide-free-detector-ab242192--sandwich-elisa-img52655.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Prostate Specific Antigen antibody [EPR22271-81] - BSA and Azide free (Detector) (AB242192)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
| Rhesus monkey | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rhesus monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Alternative names
APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin
Recommended products
Rabbit Recombinant Monoclonal Prostate Specific Antigen antibody. Carrier free. Suitable for sELISA and reacts with Human, Rhesus monkey samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22271-81
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242192 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Total PSA. The recommended pair for sandwich ELISA is:
Capture: Anti-Prostate Specific Antigen antibody [EPR22271-97] - BSA and Azide free (Capture) ab242191, Human Total PSA Capture Antibody (unconjugated)
Detector: ab242192, Human Total PSA Detector Antibody (unconjugated)
The reference range value is 226.6 - 14500 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
- Biological function summary
PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.
- Pathways
PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.
- Associated diseases and disorders
PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Prostate Specific Antigen antibody [EPR22271-81] - BSA and Azide free (Detector) (ab242192), expandable thumbnail](https://content.abcam.com/products/images/prostate-specific-antigen-antibody-epr22271-81-bsa-and-azide-free-detector-ab242192--sandwich-elisa-img52655.jpg "Sandwich ELISA - Anti-Prostate Specific Antigen antibody [EPR22271-81] - BSA and Azide free (Detector) (ab242192)")
Sandwich ELISA - Anti-Prostate Specific Antigen antibody [EPR22271-81] - BSA and Azide free (Detector) (ab242192)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human PSA ELISA Kit ab264615).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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