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AB224799

Anti-Prostate Specific Antigen antibody [SP317]

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(4 Publications)

Rabbit Recombinant Monoclonal Prostate Specific Antigen antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (AB224799)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (AB224799)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using ab224799 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (AB224799)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (AB224799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224799 for 10 mins at room temperature.

Western blot - Anti-Prostate Specific Antigen antibody [SP317] (AB224799)
  • WB

Supplier Data

Western blot - Anti-Prostate Specific Antigen antibody [SP317] (AB224799)

All lanes:

Western blot - Anti-Prostate Specific Antigen antibody [SP317] (ab224799) at 1/100 dilution

All lanes:

LNCaP (human prostate cancer cell line) cell lysate

Predicted band size: 29 kDa

false

  • Carrier free

    Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP317

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.099% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
See full target information KLK3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Veterinary medicine and science 11:e70470 PubMed40552403

2025

Co-Occurrence of Ovarian Dysgerminoma-Inducing Gonadoblastoma and Two Distinct Mammary Carcinomas in a Dog: A Case Report and Review of the Literature.

Applications

Unspecified application

Species

Unspecified reactive species

Diba Golchin,Farhang Sasani,Saeed Farzad-Mohajeri,Mohsen Tovhidifar,Iradj Ashrafi Tamai

Journal for immunotherapy of cancer 13: PubMed40121032

2025

Inhibition of stromal MAOA leading activation of WNT5A enhance prostate cancer immunotherapy by involving the transition of cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhite Zhao,Yaohua Hu,Hui Li,Tong Lu,Xinglin He,Yifan Ma,Minli Huang,Mengyao Li,Lijun Yang,Changhong Shi

Asian journal of andrology 24:525-532 PubMed35042311

2022

Immunohistochemical analysis of PD-L1 and tumor-infiltrating immune cells expression in the tumor microenvironment of primary signet ring cell carcinoma of the prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Liang Teng,Xin-Rui Yang,Shuang Wen,Zhi-Hong Dai,Hong-Long Wang,Tian-Qing Liu,Liang Wang,Bo Fan,Zhi-Yu Liu

Oncology letters 23:6 PubMed34820005

2021

Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Katia Cheaito,Hisham F Bahmad,Ola Hadadeh,Hiba Msheik,Alissar Monzer,Farah Ballout,Christelle Dagher,Talar Telvizian,Nour Saheb,Ayman Tawil,Marwan El-Sabban,Albert El-Hajj,Deborah Mukherji,Mohamed Al-Sayegh,Wassim Abou-Kheir
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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