Skip to main content

Rabbit Recombinant Monoclonal Prostate Specific Antigen antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free (AB240982), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free (AB240982), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Tested

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Alternative names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

Recommended products

Rabbit Recombinant Monoclonal Prostate Specific Antigen antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
SP317
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240982 is the carrier-free version of Anti-Prostate Specific Antigen antibody [SP317] ab224799.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.

Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

Associated diseases and disorders

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free (ab240982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free (ab240982)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with Anti-Prostate Specific Antigen antibody [SP317] ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-Prostate Specific Antigen antibody [SP317] ab224799 for 10 mins at room temperature.

    This image was generated using Anti-Prostate Specific Antigen antibody [SP317] ab224799, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free (ab240982), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] - BSA and Azide free (ab240982)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using Anti-Prostate Specific Antigen antibody [SP317] ab224799 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Prostate Specific Antigen antibody [SP317] ab224799).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com