JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231551

Anti-Prosurfactant Protein B antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal PSPB antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Sftpb aa 1-200.

View Alternative Names

Sftp3, Sftpb, Pulmonary surfactant-associated protein B, SP-B, Pulmonary surfactant-associated proteolipid SPL(Phe)

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein B antibody (AB231551)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein B antibody (AB231551)

Paraffin-embedded mouse lung tissue stained for Prosurfactant Protein B using ab231551 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein B antibody (AB231551)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein B antibody (AB231551)

Paraffin-embedded mouse liver tissue stained for Prosurfactant Protein B using ab231551 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Prosurfactant Protein B antibody (AB231551)
  • WB

Supplier Data

Western blot - Anti-Prosurfactant Protein B antibody (AB231551)

All lanes:

Western blot - Anti-Prosurfactant Protein B antibody (ab231551) at 2 µg/mL

All lanes:

Mouse liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-Prosurfactant Protein B antibody (AB231551)
  • WB

Supplier Data

Western blot - Anti-Prosurfactant Protein B antibody (AB231551)

All lanes:

Western blot - Anti-Prosurfactant Protein B antibody (ab231551) at 2 µg/mL

All lanes:

Recombinant mouse Prosurfactant Protein B protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Sftpb aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P50405

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231551 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prosurfactant Protein B also known as surfactant protein B (SP-B) is essential for pulmonary function. It is a hydrophobic protein with an approximate mass of 8 kDa after processing from its precursor form which is larger. The protein is expressed predominantly in type II alveolar cells in the lungs and is found in lamellar bodies. SP-B functions mechanically by reducing surface tension in the alveoli facilitating efficient gaseous exchange and lung compliance.
Biological function summary

SP-B plays a significant role in the formation and stabilization of pulmonary surfactant film. It acts in conjunction with phospholipids to form a protein-lipid complex. This complex is critical for lowering surface tension at the air-liquid interface in the alveoli preventing lung collapse during exhalation. SP-B integrates lipid components into the surfactant matrix and enhances its spreading and surface activity.

Pathways

SP-B serves an integral part in lung surfactant metabolism and the phospholipid metabolic pathway. These pathways ensure the homeostasis and turnover of surfactant materials. Proteins such as surfactant proteins A (SP-A) and C (SP-C) are also involved in these pathways coordinating with SP-B to maintain surfactant function and lung physiology. Together these proteins help maintain the balance necessary for proper respiratory mechanics.

Deficiency or mutation in SP-B is associated with respiratory distress syndrome (RDS) and interstitial lung diseases. These conditions manifest due to compromised surfactant function leading to alveolar collapse. SP-B mutations can disrupt the normal surfactant pathway leading to insufficient lung function. Disorders like these often involve SP-C as well with both proteins affected in cases of congenital surfactant deficiency further impacting pulmonary health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
See full target information Sftpb
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 113:105626 PubMed40023045

2025

Bi-allelic LAMP3 variants in childhood interstitial lung disease: a surfactant-related disease.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Louvrier,Tifenn Desroziers,Yohan Soreze,Martha Delgado Rodriguez,Lucie Thomas,Valérie Nau,Florence Dastot-Le Moal,Jonathan A Bernstein,F Sessions Cole,Markus Damme,Anthony Fischer,Matthias Griese,Daniel Hinds,Laura Keehan,Carlos Milla,Hadhud Mohammad,Jonathan Rips,Jennifer A Wambach,Daniel J Wegner,Serge Amselem,Marie Legendre,Irina Giurgea,Sonia Athina Karabina,Oded Breuer,Aurore Coulomb l'Herminé,Nadia Nathan

International journal of nanomedicine 18:641-657 PubMed36789391

2023

Gestational Diabetes Mellitus Impedes Fetal Lung Development Through Exosome-Dependent Crosstalk Between Trophoblasts and Lung Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pengzheng Chen,Mengqi Gu,Shuting Wan,Xiaotong Jiang,Fengyuan Zhang,Yuchen Li,Qian Zhou,Yuan Lu,Lei Li,Xietong Wang

Journal of neuroinflammation 18:64 PubMed33653377

2021

Recruitment of γδ T cells to the lesion via the CCL2/CCR2 signaling after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Xu,Feng Zhang,Min-Min Chang,Cheng Zhong,Cheng-Hong Sun,Hao-Ran Zhu,Jing-Chun Yao,Zhi-Zhong Li,Si-Tao Li,Wen-Cai Zhang,Guo-Dong Sun
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB90716

Anti-Prosurfactant Protein C antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (AB90716)

  • 4
  • 12
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB76013

Anti-TTF1/Nkx2-1 antibody [EP1584Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

  • 5
  • 17
Primary Antibodies

AB115791

Anti-Surfactant Protein A/PSAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (AB115791)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com