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AB196677

Anti-Prosurfactant Protein C antibody

1

(1 Review)

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(3 Publications)

Rabbit Polyclonal Prosurfactant Protein C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SFTPC.

View Alternative Names

SFTP2, SFTPC, Surfactant protein C, SP-C, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (AB196677)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (AB196677)

Immunofluorescent analysis of U2OS cells labeling SFTPC with ab196677 at 1/50 dilution. Lower image with DAPI for nuclear staining.

Western blot - Anti-Prosurfactant Protein C antibody (AB196677)
  • WB

Supplier Data

Western blot - Anti-Prosurfactant Protein C antibody (AB196677)

All lanes:

Western blot - Anti-Prosurfactant Protein C antibody (ab196677) at 1/500 dilution

Lane 1:

Jurkat cell extract

Lane 2:

U937 cell extract

Lane 3:

Raji cell extract

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SFTPC.

P11686

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prosurfactant Protein C (Pro-SP-C) often referred to as SPC or by its abbreviated form SP-C is a hydrophobic peptide critical for lung function. This protein has a mass of approximately 21 kDa and is expressed prominently in the alveolar type II cells of the lung tissue. Beyond its full-length variant various pro forms such as proSP-C are studied for their roles during protein maturation and trafficking. These pro forms are essential for producing the mature functional SP-C a critical component of lung surfactant which reduces surface tension in alveoli and prevents lung collapse.
Biological function summary

In surfactant homeostasis Prosurfactant Protein C associates with surfactant protein complexes where it interacts with phospholipids like phosphatidylcholine to stabilize the alveolar structures. As part of this complex it aids in reducing surface tension at the air-liquid interface in the alveoli and contributes to the host defense system by fostering pathogen clearance and modulating inflammation. The intricate processing of Pro-SP-C into its mature form involves fucosylation and other post-translational modifications which are key for functional surfactant activity.

Pathways

The role of Prosurfactant Protein C is integral to the surfactant metabolism pathway affecting lung function and homeostasis. This pathway is important for proper respiratory mechanics and is closely linked to other surfactant proteins like SP-B and SP-D. These proteins work together within the lipid-protein matrix of the surfactant to ensure proper alveolar deflation and reinflation during respiration. Disruption within these pathways can have significant impact on lung health and respiratory physiology.

Mutations or dysfunctions in Prosurfactant Protein C are linked to interstitial lung diseases including pulmonary alveolar proteinosis and idiopathic pulmonary fibrosis. These conditions often result from misfolded proteins or improper surfactant metabolism leading to compromised lung function. Connections with disorders like these highlight interactions between Prosurfactant Protein C and C-reactive protein uncovering underlying inflammatory processes. Therapy and research often target these protein interactions and modifications as understanding them holds potential for strategic intervention in surfactant-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
See full target information SFTPC
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35993367

2022

Increased expression and accumulation of GDF15 in IPF extracellular matrix contribute to fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Agata Radwanska,Christopher Travis Cottage,Antonio Piras,Catherine Overed-Sayer,Carina Sihlbom,Ramachandramouli Budida,Catherine Wrench,Jane Connor,Susan Monkley,Petra Hazon,Holger Schluter,Matthew J Thomas,Cory M Hogaboam,Lynne A Murray

Annals of translational medicine 9:563 PubMed33987261

2021

Nogo-B promotes epithelial-mesenchymal transition in lung fibrosis via PERK branch of the endoplasmic reticulum stress pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhu,Meng Yang,Xue-Hui Li,Wu-Jian Xu,Wei Gao,Yu-Han Chen,Jian-Dong Li,Qiang Li

Nature communications 10:1985 PubMed31064987

2019

Regeneration of severely damaged lungs using an interventional cross-circulation platform.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon A Guenthart,John D O'Neill,Jinho Kim,Dawn Queen,Scott Chicotka,Kenmond Fung,Michael Simpson,Rachel Donocoff,Michael Salna,Charles C Marboe,Katherine Cunningham,Susan P Halligan,Holly M Wobma,Ahmed E Hozain,Alexander Romanov,Gordana Vunjak-Novakovic,Matthew Bacchetta
View all publications
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Product promise

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