Anti-Prosurfactant Protein C antibody ab90716 is a rabbit polyclonal antibody that is used in Prosurfactant Protein C western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.
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- Journal of gastroenterology 59:95-1082023Marked intestinal trans-differentiation by autoimmune gastritis along with ectopic pancreatic and pulmonary trans-differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChihiro Takeuchi,Junichi Sato,Nobutake Yamamichi,Natsuko Kageyama-Yahara,Akiko Sasaki,Takemi Akahane,Rika Aoki,Shigemi Nakajima,Masayoshi Ito,Mitsue Yamamichi,Yu-Yu Liu,Nobuyuki Sakuma,Yu Takahashi,Yoshiki Sakaguchi,Yosuke Tsuji,Kouhei Sakurai,Shuta Tomida,Keiko Niimi,Toshikazu Ushijima,Mitsuhiro FujishiroPubMed 37962678
- Cellular & molecular biology letters 28:912023Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Ling,Shiyou Wei,Dandan Ling,Siqi Cao,Rui Chang,Qiuyun Wang,Zhize YuanPubMed 37946128
- Nature communications 14:72162023Single cell spatial analysis reveals inflammatory foci of immature neutrophil and CD8 T cells in COVID-19 lungs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPraveen Weeratunga,Laura Denney,Joshua A Bull,Emmanouela Repapi,Martin Sergeant,Rachel Etherington,Chaitanya Vuppussetty,Gareth D H Turner,Colin Clelland,Jeongmin Woo,Amy Cross,Fadi Issa,Carlos Eduardo de Andrea,Ignacio Melero Bermejo,David Sims,Simon McGowan,Yasemin-Xiomara Zurke,David J Ahern,Eddie C Gamez,Justin Whalley,Duncan Richards,Paul Klenerman,Claudia Monaco,Irina A Udalova,Tao Dong,Agne Antanaviciute,Graham Ogg,Julian C Knight,Helen M Byrne,Stephen Taylor,Ling-Pei HoPubMed 37940670
- JCI insight 8:2023Alveolar repair following LPS-induced injury requires cell-ECM interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer Ms Sucre,Fabian Bock,Nicholas M Negretti,John T Benjamin,Peter M Gulleman,Xinyu Dong,Kimberly T Ferguson,Christopher S Jetter,Wei Han,Yang Liu,Seunghyi Kook,Jason J Gokey,Susan H Guttentag,Jonathan A Kropski,Timothy S Blackwell,Roy Zent,Erin J PlosaPubMed 37279065
- Respiratory research 24:1822023Identification of a novel role for TL1A/DR3 deficiency in acute respiratory distress syndrome that exacerbates alveolar epithelial disruption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Zhang,Jianning Zhang,Jintao Zhang,Xiang Ji,Qian Qi,Jiawei Xu,Yun Pan,Xiaofei Liu,Fang Sun,Rong Zeng,Liang DongPubMed 37434162
- Aging 15:4625-46412023Short telomeres in alveolar type II cells associate with lung fibrosis in post COVID-19 patients with cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaula Martínez,Raúl Sánchez-Vazquez,Arpita Saha,Maria S Rodriguez-Duque,Sara Naranjo-Gonzalo,Joy S Osorio-Chavez,Ana V Villar-Ramos,Maria A BlascoPubMed 37294548
- Scientific reports 13:90442023Overexpression of fatty acid synthase attenuates bleomycin induced lung fibrosis by restoring mitochondrial dysfunction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyesun Shin,Shinhee Park,Jisu Hong,Ae-Rin Baek,Junehyuk Lee,Do-Jin Kim,An-Soo Jang,Su Sie Chin,Sung Hwan Jeong,Sung-Woo ParkPubMed 37270622
- Scientific reports 13:88012023Alveolar epithelial cells are competent producers of interstitial extracellular matrix with disease relevant plasticity in a human in vitro 3D model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOskar Rosmark,Måns Kadefors,Göran Dellgren,Christofer Karlsson,Anders Ericsson,Sandra Lindstedt,Johan Malmström,Oskar Hallgren,Anna-Karin Larsson-Callerfelt,Gunilla Westergren-ThorssonPubMed 37258541
- Cell reports 42:1124512023Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian L Cardenas-Diaz,Derek C Liberti,John P Leach,Apoorva Babu,Jonathan Barasch,Tian Shen,Maria A Diaz-Miranda,Su Zhou,Yun Ying,Danielle A Callaway,Michael P Morley,Edward E MorriseyPubMed 37119134
- Development (Cambridge, England) 150:2023Sp3 is essential for normal lung morphogenesis and cell cycle progression during mouse embryonic development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyssa M McCoy,Omar Lakhdari,Sayane Shome,Kaitlin Caoili,Gilberto E Hernandez,Nima Aghaeepour,Lindsay D Butcher,Kathleen Fisch,Lawrence S PrincePubMed 36762637
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Mouse | Tested | Expected | Expected | Expected |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Alternative names
Pulmonary surfactant-associated protein C, SP-C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5, SFTPC, SFTP2
Recommended products
Anti-Prosurfactant Protein C antibody ab90716 is a rabbit polyclonal antibody that is used in Prosurfactant Protein C western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab211326.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prosurfactant Protein C (Pro-SP-C) often referred to as SPC or by its abbreviated form SP-C is a hydrophobic peptide critical for lung function. This protein has a mass of approximately 21 kDa and is expressed prominently in the alveolar type II cells of the lung tissue. Beyond its full-length variant various pro forms such as proSP-C are studied for their roles during protein maturation and trafficking. These pro forms are essential for producing the mature functional SP-C a critical component of lung surfactant which reduces surface tension in alveoli and prevents lung collapse.
- Biological function summary
In surfactant homeostasis Prosurfactant Protein C associates with surfactant protein complexes where it interacts with phospholipids like phosphatidylcholine to stabilize the alveolar structures. As part of this complex it aids in reducing surface tension at the air-liquid interface in the alveoli and contributes to the host defense system by fostering pathogen clearance and modulating inflammation. The intricate processing of Pro-SP-C into its mature form involves fucosylation and other post-translational modifications which are key for functional surfactant activity.
- Pathways
The role of Prosurfactant Protein C is integral to the surfactant metabolism pathway affecting lung function and homeostasis. This pathway is important for proper respiratory mechanics and is closely linked to other surfactant proteins like SP-B and SP-D. These proteins work together within the lipid-protein matrix of the surfactant to ensure proper alveolar deflation and reinflation during respiration. Disruption within these pathways can have significant impact on lung health and respiratory physiology.
- Associated diseases and disorders
Mutations or dysfunctions in Prosurfactant Protein C are linked to interstitial lung diseases including pulmonary alveolar proteinosis and idiopathic pulmonary fibrosis. These conditions often result from misfolded proteins or improper surfactant metabolism leading to compromised lung function. Connections with disorders like these highlight interactions between Prosurfactant Protein C and C-reactive protein uncovering underlying inflammatory processes. Therapy and research often target these protein interactions and modifications as understanding them holds potential for strategic intervention in surfactant-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody (ab90716)
IHC image of Prosurfactant Protein C staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab90716, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunoprecipitation - Anti-Prosurfactant Protein C antibody (ab90716)
Prosurfactant Protein C was immunoprecipitated using 0.5mg Mouse Lung tissue, 5μg of Rabbit polyclonal to Prosurfactant Protein C and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90716.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 23kDa; Prosurfactant Protein C
All lanes: Immunoprecipitation - Anti-Prosurfactant Protein C antibody (ab90716)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 20min
Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (ab90716)
ab90716 staining Prosurfactant Protein C in A549 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab90716 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Prosurfactant Protein C antibody (ab90716)
All lanes: Western blot - Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/mL
Lane 1: Lung (Human) Tissue Lysate at 10 µg
Lane 2: Lung (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 25 kDa, 28 kDa, 90 kDa
Exposure time: 3min
Western blot - Anti-Prosurfactant Protein C antibody (ab90716)
All lanes: Western blot - Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/mL
All lanes: Lung (Human) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 25 kDa, 28 kDa, 45 kDa
Exposure time: 2min
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