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AB211326

Anti-Prosurfactant Protein C antibody [EPR19839]

5

(3 Reviews)

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(66 Publications)

Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326) is a rabbit monoclonal antibody detecting Prosurfactant Protein C in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

View Alternative Names

Sftp2, Sftpc, Surfactant protein C, SP-C, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID : 15186480].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID : 23967208].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : no staining on mouse spleen. [PMID : 15186480]. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)
  • IP

Unknown

Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)

SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Mouse lung lysate, 10ug (Input).

Lane 2 : ab211326 IP in mouse lung lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

The two bands detected correspond to 22 kda and 21 kda isoforms.

All lanes:

Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

Predicted band size: 21 kDa

Observed band size: 21 kDa,22 kDa

false

Western blot - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)
  • WB

Supplier Data

Western blot - Anti-Prosurfactant Protein C antibody [EPR19839] (AB211326)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : mouse spleen. [PMID : 15186480].

All lanes:

Western blot - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa,89 kDa

Observed band size: 100 kDa,21 kDa,22 kDa

false

Exposure time: 5s

  • Carrier free

    Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free

  • HRP

    HRP Anti-Prosurfactant Protein C antibody [EPR19839]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19839

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IP, IHC-P, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples.

What is the molecular weight of Prosurfactant Protein C?
Anti-Prosurfactant Protein C [EPR19839] (ab211326) specifically detects a band for Prosurfactant Protein C (UniProt: P21841) at a molecular weight of 21kDa.

Trusted by the scientific community
Anti-Prosurfactant Protein C [EPR19839] (ab211326) was first used in a scientific publication in 2016 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19839] also available for your convenience: ab211326, Carrier free - ab222929, Carrier free - ab271991, HRP - ab314245

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prosurfactant Protein C (Pro-SP-C) often referred to as SPC or by its abbreviated form SP-C is a hydrophobic peptide critical for lung function. This protein has a mass of approximately 21 kDa and is expressed prominently in the alveolar type II cells of the lung tissue. Beyond its full-length variant various pro forms such as proSP-C are studied for their roles during protein maturation and trafficking. These pro forms are essential for producing the mature functional SP-C a critical component of lung surfactant which reduces surface tension in alveoli and prevents lung collapse.
Biological function summary

In surfactant homeostasis Prosurfactant Protein C associates with surfactant protein complexes where it interacts with phospholipids like phosphatidylcholine to stabilize the alveolar structures. As part of this complex it aids in reducing surface tension at the air-liquid interface in the alveoli and contributes to the host defense system by fostering pathogen clearance and modulating inflammation. The intricate processing of Pro-SP-C into its mature form involves fucosylation and other post-translational modifications which are key for functional surfactant activity.

Pathways

The role of Prosurfactant Protein C is integral to the surfactant metabolism pathway affecting lung function and homeostasis. This pathway is important for proper respiratory mechanics and is closely linked to other surfactant proteins like SP-B and SP-D. These proteins work together within the lipid-protein matrix of the surfactant to ensure proper alveolar deflation and reinflation during respiration. Disruption within these pathways can have significant impact on lung health and respiratory physiology.

Mutations or dysfunctions in Prosurfactant Protein C are linked to interstitial lung diseases including pulmonary alveolar proteinosis and idiopathic pulmonary fibrosis. These conditions often result from misfolded proteins or improper surfactant metabolism leading to compromised lung function. Connections with disorders like these highlight interactions between Prosurfactant Protein C and C-reactive protein uncovering underlying inflammatory processes. Therapy and research often target these protein interactions and modifications as understanding them holds potential for strategic intervention in surfactant-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
See full target information Sftpc
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Publications (66)

Recent publications for all applications. Explore the full list and refine your search

Biological procedures online 27:21 PubMed40524168

2025

Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening.

Applications

Unspecified application

Species

Unspecified reactive species

Yajing Liu,Jingyuan Ning,Donglai Wang

Journal of nanobiotechnology 23:339 PubMed40340852

2025

Dual-responsive nanoparticles targeting ACE-II senescence for therapeutic mitigation of acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Gao,Fushuang Zheng,Zhiling Fu,Wei Wang

Nature communications 16:3429 PubMed40210885

2025

Genetic modulation of rare earth nanoparticle biotransformation shapes biological outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Tian,Di Wu,Xiao Gou,Ruibin Li,Xiaowei Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2405434 PubMed40119620

2025

Inhalable Hsa-miR-30a-3p Liposomes Attenuate Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liu,Kristen D Popowski,Christina M Eckhardt,Weihang Zhang,Junlang Li,Yujia Jing,Dylan Silkstone,Elizabeth Belcher,Megan Cislo,Shiqi Hu,Halle Lutz,Asma Ghodsi,Mengrui Liu,Phuong-Uyen C Dinh,Ke Cheng

The EMBO journal 44:1804-1828 PubMed39930268

2025

Organoid modeling reveals the tumorigenic potential of the alveolar progenitor cell state.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyun Li,Susanna M Dang,Shreoshi Sengupta,Paul Schurmann,Antonella F M Dost,Aaron L Moye,Maria F Trovero,Sidrah Ahmed,Margherita Paschini,Preetida J Bhetariya,Roderick Bronson,Shannan J Ho Sui,Carla F Kim

Stem cell reports 20:102373 PubMed39642874

2024

A versatile in vivo platform for reversible control of transgene expression in adult tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Jumpei Taguchi,Yosuke Yamada,Sho Ohta,Fumie Nakasuka,Takuya Yamamoto,Manabu Ozawa,Yasuhiro Yamada

Clinical cancer research : an official journal of the American Association for Cancer Research 31:414-429 PubMed39540841

2024

Aberrant Activation of Wound-Healing Programs within the Metastatic Niche Facilitates Lung Colonization by Osteosarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

James B Reinecke,Leyre Jimenez Garcia,Amy C Gross,Maren Cam,Matthew V Cannon,Matthew J Gust,Jeffrey P Sheridan,Berkley E Gryder,Ruben Dries,Ryan D Roberts

Nature communications 15:8530 PubMed39358385

2024

Interleukin-11 causes alveolar type 2 cell dysfunction and prevents alveolar regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Ng,Kevin Y Huang,Chee Jian Pua,Sivakumar Viswanathan,Wei-Wen Lim,Fathima F Kuthubudeen,Yu-Ning Liu,An An Hii,Benjamin L George,Anissa A Widjaja,Enrico Petretto,Stuart A Cook

Cell reports. Medicine 5:101732 PubMed39255796

2024

Lipolysis engages CD36 to promote ZBP1-mediated necroptosis-impairing lung regeneration in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Jiazhen Wang,Ru Wang,Yicun Li,Jiahui Huang,Yang Liu,Jiayi Wang,Peng Xian,Yuanhang Zhang,Yanmei Yang,Haojian Zhang,Jiansheng Li

Redox biology 74:103231 PubMed38861835

2024

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Yu Fu,Jiameng Gao,Qingqing Zhang,Nan Zhang,Zhiyuan Zhang,Xuemei Jiang,Chang Chen,Zongmei Wen
View all publications
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