AB271991

Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free

Name: Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (ab271991) | Abcam
Brand: Abcam Limited
SKU: AB271991
Price: 905 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Prosurfactant Protein C antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse samples.

View Alternative Names

Sftp2, Sftpc, Surfactant protein C, SP-C, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID : 15186480].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID : 23967208].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : no staining on mouse spleen. [PMID : 15186480]. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

Unknown

Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Mouse lung lysate, 10ug (Input).

Lane 2 : ab211326 IP in mouse lung lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

The banding pattern represents pro- and mature forms of SFTPC.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

All lanes:

Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] (<a href='/en-us/products/primary-antibodies/prosurfactant-protein-c-antibody-epr19839-ab211326'>ab211326</a>)

Predicted band size: 21 kDa

Observed band size: 21 kDa,22 kDa

false

Unconjugated

Anti-Prosurfactant Protein C antibody [EPR19839]
Carrier free

Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free
HRP

HRP Anti-Prosurfactant Protein C antibody [EPR19839]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19839

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IP, IHC-P, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab271991 is the carrier-free version of ab211326.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prosurfactant Protein C (Pro-SP-C) often referred to as SPC or by its abbreviated form SP-C is a hydrophobic peptide critical for lung function. This protein has a mass of approximately 21 kDa and is expressed prominently in the alveolar type II cells of the lung tissue. Beyond its full-length variant various pro forms such as proSP-C are studied for their roles during protein maturation and trafficking. These pro forms are essential for producing the mature functional SP-C a critical component of lung surfactant which reduces surface tension in alveoli and prevents lung collapse.

Biological function summary

In surfactant homeostasis Prosurfactant Protein C associates with surfactant protein complexes where it interacts with phospholipids like phosphatidylcholine to stabilize the alveolar structures. As part of this complex it aids in reducing surface tension at the air-liquid interface in the alveoli and contributes to the host defense system by fostering pathogen clearance and modulating inflammation. The intricate processing of Pro-SP-C into its mature form involves fucosylation and other post-translational modifications which are key for functional surfactant activity.

Pathways

The role of Prosurfactant Protein C is integral to the surfactant metabolism pathway affecting lung function and homeostasis. This pathway is important for proper respiratory mechanics and is closely linked to other surfactant proteins like SP-B and SP-D. These proteins work together within the lipid-protein matrix of the surfactant to ensure proper alveolar deflation and reinflation during respiration. Disruption within these pathways can have significant impact on lung health and respiratory physiology.

Mutations or dysfunctions in Prosurfactant Protein C are linked to interstitial lung diseases including pulmonary alveolar proteinosis and idiopathic pulmonary fibrosis. These conditions often result from misfolded proteins or improper surfactant metabolism leading to compromised lung function. Connections with disorders like these highlight interactions between Prosurfactant Protein C and C-reactive protein uncovering underlying inflammatory processes. Therapy and research often target these protein interactions and modifications as understanding them holds potential for strategic intervention in surfactant-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.

See full target information Sftpc

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - BSA and Azide free (AB271991)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise