JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190791

Anti-Protamine 2 antibody [EPR15738]

5

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal Protamine 2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Protamine-2, Sperm histone P2, Sperm protamine P2, PRM2

1 Images
Western blot - Anti-Protamine 2 antibody [EPR15738] (AB190791)
  • WB

Supplier Data

Western blot - Anti-Protamine 2 antibody [EPR15738] (AB190791)

All lanes:

Western blot - Anti-Protamine 2 antibody [EPR15738] (ab190791) at 1/10000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

false

  • Carrier free

    Anti-Protamine 2 antibody [EPR15738] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15738

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protamine 2 also known as PRM2 or protamine protein is a nuclear protein with a molecular mass of approximately 14 kDa. It is predominantly expressed in the testes specifically during the spermatogenesis process. Protamine 2 replaces histones in haploid phases of spermatids to ensure the proper condensation of sperm chromatin. Its structure comprises arginine-rich sequences enabling the tight packaging of DNA which is important for mature sperm functionality.
Biological function summary

Protamine 2 plays an essential role in sperm chromatin structure and stability interacting with other nucleoproteins to form a protamine-histone-DNA complex. This interaction is fundamental for the accurate packing of DNA within a sperm cell. Protamine 2 and its partners in the complex are critical for the fertility of males as they impact the genetic information's effective transmission to the next generation. The alteration of Protamine's balance affects the integrity of human sperm leading to reproductive challenges.

Pathways

Protamine 2 integrates into the spermatogenesis pathway coordinating with other proteins like Protamine 1 and transition proteins. Together they conduct transitions from histone-based chromatin to protamine-based packaging. This process is essential within the broader context of the reproductive signaling pathway which includes a range of post-translational modifications central to sperm development and maturation.

Disruptions in Protamine 2 expression link to male infertility as imbalances in Protamine 1 and 2 ratios can result in defective sperm packaging. Evidence also associates Protamine 2 dysregulation with a higher risk of sperm DNA fragmentation which can contribute to disorders such as recurrent pregnancy loss. The protein's performance and interaction with other proteins like histones and nuclear transition proteins point to its importance in maintaining genomic integrity during the paternal genetic material transmission.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
See full target information PRM2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics 20:2498859 PubMed40296586

2025

Higher sperm H3K4me3 levels are associated with idiopathic recurrent pregnancy loss.

Applications

Unspecified application

Species

Unspecified reactive species

Delna Irani,Deepshikha Arya,Deepti Tandon,Vandana Bansal,Anushree Patil,Dipty Singh

Molecular & cellular proteomics : MCP 15:3297-3320 PubMed27451424

2016

Proteoform-Specific Insights into Cellular Proteome Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Norris,Madeleine J Headlam,Keyur A Dave,David D Smith,Alexander Bukreyev,Toshna Singh,Buddhika A Jayakody,Keith J Chappell,Peter L Collins,Jeffrey J Gorman
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB66978

Anti-HSP1 / Protamine P1 antibody

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB308167

Anti-Miz1 antibody [HL1548]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Miz1 antibody [HL1548] (AB308167)

  • 0
  • 0
Primary Antibodies

AB97672

Anti-SCP3 antibody [Cor 10G11/7]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (AB97672)

  • 5
  • 10

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com