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AB240968

Anti-Protamine 2 antibody [EPR15738] - BSA and Azide free

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal Protamine 2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Protamine-2, Sperm histone P2, Sperm protamine P2, PRM2

1 Images
Western blot - Anti-Protamine 2 antibody [EPR15738] - BSA and Azide free (AB240968)
  • WB

Supplier Data

Western blot - Anti-Protamine 2 antibody [EPR15738] - BSA and Azide free (AB240968)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190791).

All lanes:

Western blot - Anti-Protamine 2 antibody [EPR15738] (<a href='/en-us/products/primary-antibodies/protamine-2-antibody-epr15738-ab190791'>ab190791</a>) at 1/10000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15738

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab240968 is the carrier-free version of ab190791.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protamine 2 also known as PRM2 or protamine protein is a nuclear protein with a molecular mass of approximately 14 kDa. It is predominantly expressed in the testes specifically during the spermatogenesis process. Protamine 2 replaces histones in haploid phases of spermatids to ensure the proper condensation of sperm chromatin. Its structure comprises arginine-rich sequences enabling the tight packaging of DNA which is important for mature sperm functionality.
Biological function summary

Protamine 2 plays an essential role in sperm chromatin structure and stability interacting with other nucleoproteins to form a protamine-histone-DNA complex. This interaction is fundamental for the accurate packing of DNA within a sperm cell. Protamine 2 and its partners in the complex are critical for the fertility of males as they impact the genetic information's effective transmission to the next generation. The alteration of Protamine's balance affects the integrity of human sperm leading to reproductive challenges.

Pathways

Protamine 2 integrates into the spermatogenesis pathway coordinating with other proteins like Protamine 1 and transition proteins. Together they conduct transitions from histone-based chromatin to protamine-based packaging. This process is essential within the broader context of the reproductive signaling pathway which includes a range of post-translational modifications central to sperm development and maturation.

Disruptions in Protamine 2 expression link to male infertility as imbalances in Protamine 1 and 2 ratios can result in defective sperm packaging. Evidence also associates Protamine 2 dysregulation with a higher risk of sperm DNA fragmentation which can contribute to disorders such as recurrent pregnancy loss. The protein's performance and interaction with other proteins like histones and nuclear transition proteins point to its importance in maintaining genomic integrity during the paternal genetic material transmission.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
See full target information PRM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:2209-2219 PubMed32186759

2020

PDGFD induces ibrutinib resistance of diffuse large B‑cell lymphoma through activation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Jin,Leiping Wang,Zhonghua Tao,Jian Zhang,Fangfang Lv,Junning Cao,Xichun Hu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com