AB140450

Anti-Proteasome 19S S4/PSMC1 antibody

Name: Anti-Proteasome 19S S4/PSMC1 antibody (ab140450) | Abcam
Brand: Abcam Limited
SKU: AB140450
Price: 535 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(2 Publications)

Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human 26S proteasome regulatory subunit 4 aa 350 to C-terminus.

View Alternative Names

26S proteasome regulatory subunit 4, P26s4, 26S proteasome AAA-ATPase subunit RPT2, Proteasome 26S subunit ATPase 1, PSMC1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

Paraffin embedded human ovarian carcinoma tissue stained for Proteasome 19S S4/PSMC1 using ab140450 at 1/1000 dilution in immunohistochemical analysis.

Unknown

Immunoprecipitation - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

Detection of Proteasome 19S S4/PSMC1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140450 at 6 μg/mg lysate for IP and 1μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Proteasome 19S S4/PSMC1 antibody (ab140450)

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

All lanes:

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab140450) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human 26S proteasome regulatory subunit 4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62191

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab140450 was affinity purified using an epitope specific to Proteasome 19S S4/PSMC1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 19S S4 also known as PSMC1 is a mechanical component of the 26S proteasome an ATP-dependent protease complex responsible for protein degradation. This protein functions as an ATPase and contributes to the unfolding of ubiquitinated proteins preparing them for translocation into the 20S core particle of the proteasome for degradation. S4/PSMC1 has a molecular mass of approximately 49 kDa and is expressed ubiquitously across various cell types reflecting its essential role in cellular protein homeostasis.

Biological function summary

PSMC1 functions as an integral part of the 19S regulatory particle of the 26S proteasome complex. This complex facilitates the recognition unfolding and translocation of polyubiquitinated proteins into the proteolytic chamber of the 20S core particle for degradation. This activity plays a significant role in regulating intracellular protein concentrations thereby controlling processes like cell cycle progression apoptosis and responses to oxidative stress. PSMC1’s activity ensures a clean cellular environment by removing damaged or nonfunctional proteins.

Pathways

Functions of PSMC1 integrate into the ubiquitin-proteasome pathway and the cell cycle control pathway. In the ubiquitin-proteasome pathway PSMC1 collaborates with other ATPase subunits and regulatory proteins helping to maintain protein degradation balance and works closely with ubiquitin-conjugating enzymes responsible for tagging proteins for degradation. In the cell cycle control pathway the degradation of cyclins and cyclin-dependent kinase inhibitors by the proteasome including PSMC1 regulates cell cycle transitions and ensures proper cell division and function.

Alterations in PSMC1 function or expression can link to disorders such as cancer and neurodegenerative diseases. In cancer the dysregulation of PSMC1 can affect cellular proliferation by disrupting regulated protein degradation leading to uncontrolled cell growth. In neurodegenerative diseases such as Parkinson's impaired proteasome function including the activity of PSMC1 may lead to the accumulation of misfolded proteins contributing to neuronal cell death. Connections to other proteins like cyclins in cancer or alpha-synuclein in Parkinson's enhance the consequences of dysfunctional PSMC1 activity in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

See full target information 26S proteasome regulatory subunit 4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabk2252 PubMed35675410

2022

Genetic and pharmacologic proteasome augmentation ameliorates Alzheimer's-like pathology in mouse and fly APP overexpression models.

Applications

Unspecified application

Species

Unspecified reactive species

E Sandra Chocron,Erin Munkácsy,Harper S Kim,Przemyslaw Karpowicz,Nisi Jiang,Candice E Van Skike,Nicholas DeRosa,Andy Q Banh,Juan P Palavicini,Paweł Wityk,Leszek Kalinowski,Veronica Galvan,Pawel A Osmulski,Elzbieta Jankowska,Maria Gaczynska,Andrew M Pickering

Human molecular genetics 25:1979-1989 PubMed26962150

2016

A small-molecule Nrf1 and Nrf2 activator mitigates polyglutamine toxicity in spinal and bulbar muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Laura C Bott,Nisha M Badders,Ke-Lian Chen,George G Harmison,Elaine Bautista,Charles C-Y Shih,Masahisa Katsuno,Gen Sobue,J Paul Taylor,Nico P Dantuma,Kenneth H Fischbeck,Carlo Rinaldi

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Proteasome 19S S4/PSMC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

Immunoprecipitation - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB140450)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise