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Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765), expandable thumbnail
  • Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765), expandable thumbnail
  • Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P62191

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Chicken
Predicted
Predicted
Predicted
Drosophila melanogaster
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Chicken, Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Drosophila melanogaster

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Alternative names

Recommended products

Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P62191
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Proteasome 19S S4 also known as PSMC1 is a mechanical component of the 26S proteasome an ATP-dependent protease complex responsible for protein degradation. This protein functions as an ATPase and contributes to the unfolding of ubiquitinated proteins preparing them for translocation into the 20S core particle of the proteasome for degradation. S4/PSMC1 has a molecular mass of approximately 49 kDa and is expressed ubiquitously across various cell types reflecting its essential role in cellular protein homeostasis.

Biological function summary

PSMC1 functions as an integral part of the 19S regulatory particle of the 26S proteasome complex. This complex facilitates the recognition unfolding and translocation of polyubiquitinated proteins into the proteolytic chamber of the 20S core particle for degradation. This activity plays a significant role in regulating intracellular protein concentrations thereby controlling processes like cell cycle progression apoptosis and responses to oxidative stress. PSMC1’s activity ensures a clean cellular environment by removing damaged or nonfunctional proteins.

Pathways

Functions of PSMC1 integrate into the ubiquitin-proteasome pathway and the cell cycle control pathway. In the ubiquitin-proteasome pathway PSMC1 collaborates with other ATPase subunits and regulatory proteins helping to maintain protein degradation balance and works closely with ubiquitin-conjugating enzymes responsible for tagging proteins for degradation. In the cell cycle control pathway the degradation of cyclins and cyclin-dependent kinase inhibitors by the proteasome including PSMC1 regulates cell cycle transitions and ensures proper cell division and function.

Associated diseases and disorders

Alterations in PSMC1 function or expression can link to disorders such as cancer and neurodegenerative diseases. In cancer the dysregulation of PSMC1 can affect cellular proliferation by disrupting regulated protein degradation leading to uncontrolled cell growth. In neurodegenerative diseases such as Parkinson's impaired proteasome function including the activity of PSMC1 may lead to the accumulation of misfolded proteins contributing to neuronal cell death. Connections to other proteins like cyclins in cancer or alpha-synuclein in Parkinson's enhance the consequences of dysfunctional PSMC1 activity in these diseases.

Product promise

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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765)

    Immunohistochemical analysis of human testis tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in cells in seminiferous duct.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765)

    Immunohistochemical analysis of human pancreas tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in the islets of Langerhan's.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765)

    Immunohistochemical analysis of human prostate tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in glandular cells.

  • Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765), expandable thumbnail

    Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765)

    All lanes: Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) at 1/250 dilution

    All lanes: EFO-21 whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 49 kDa

  • Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765), expandable thumbnail

    Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765)

    All lanes: Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) at 1/250 dilution

    Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lane 2: NBT-II whole cell lysate

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Proteasome 19S S4/PSMC1 (green) using ab223765 at 4 μg/ml in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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