AB223765

Anti-Proteasome 19S S4/PSMC1 antibody

Name: Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) | Abcam
Brand: Abcam Limited
SKU: AB223765

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(2 Publications)

Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus.

View Alternative Names

26S proteasome regulatory subunit 4, P26s4, 26S proteasome AAA-ATPase subunit RPT2, Proteasome 26S subunit ATPase 1, PSMC1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Immunohistochemical analysis of human pancreas tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in the islets of Langerhan's.

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Proteasome 19S S4/PSMC1 (green) using ab223765 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Immunohistochemical analysis of human prostate tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Immunohistochemical analysis of human testis tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in cells in seminiferous duct.

Supplier Data

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

All lanes:

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II whole cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

Predicted band size: 49 kDa

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Supplier Data

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

All lanes:

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) at 1/250 dilution

All lanes:

EFO-21 whole cell lysate

Predicted band size: 49 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62191

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 19S S4 also known as PSMC1 is a mechanical component of the 26S proteasome an ATP-dependent protease complex responsible for protein degradation. This protein functions as an ATPase and contributes to the unfolding of ubiquitinated proteins preparing them for translocation into the 20S core particle of the proteasome for degradation. S4/PSMC1 has a molecular mass of approximately 49 kDa and is expressed ubiquitously across various cell types reflecting its essential role in cellular protein homeostasis.

Biological function summary

PSMC1 functions as an integral part of the 19S regulatory particle of the 26S proteasome complex. This complex facilitates the recognition unfolding and translocation of polyubiquitinated proteins into the proteolytic chamber of the 20S core particle for degradation. This activity plays a significant role in regulating intracellular protein concentrations thereby controlling processes like cell cycle progression apoptosis and responses to oxidative stress. PSMC1’s activity ensures a clean cellular environment by removing damaged or nonfunctional proteins.

Pathways

Functions of PSMC1 integrate into the ubiquitin-proteasome pathway and the cell cycle control pathway. In the ubiquitin-proteasome pathway PSMC1 collaborates with other ATPase subunits and regulatory proteins helping to maintain protein degradation balance and works closely with ubiquitin-conjugating enzymes responsible for tagging proteins for degradation. In the cell cycle control pathway the degradation of cyclins and cyclin-dependent kinase inhibitors by the proteasome including PSMC1 regulates cell cycle transitions and ensures proper cell division and function.

Alterations in PSMC1 function or expression can link to disorders such as cancer and neurodegenerative diseases. In cancer the dysregulation of PSMC1 can affect cellular proliferation by disrupting regulated protein degradation leading to uncontrolled cell growth. In neurodegenerative diseases such as Parkinson's impaired proteasome function including the activity of PSMC1 may lead to the accumulation of misfolded proteins contributing to neuronal cell death. Connections to other proteins like cyclins in cancer or alpha-synuclein in Parkinson's enhance the consequences of dysfunctional PSMC1 activity in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

See full target information 26S proteasome regulatory subunit 4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2179 PubMed35449133

2022

Golgi apparatus-targeted aggregation-induced emission luminogens for effective cancer photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Minglun Liu,Yuncong Chen,Yan Guo,Hao Yuan,Tongxiao Cui,Shankun Yao,Suxing Jin,Huanhuan Fan,Chengjun Wang,Ran Xie,Weijiang He,Zijian Guo

Frontiers in cell and developmental biology 9:650817 PubMed34055780

2021

Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Ibañez-Vega,Felipe Del Valle,Juan José Sáez,Fanny Guzman,Jheimmy Diaz,Andrea Soza,María Isabel Yuseff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 19S S4/PSMC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (AB223765)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise