Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Chicken | Predicted | Predicted | Predicted |
Drosophila melanogaster | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Drosophila melanogaster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Drosophila melanogaster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Drosophila melanogaster | Dilution info - | Notes - |
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Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
26S proteasome regulatory subunit 4, P26s4, 26S proteasome AAA-ATPase subunit RPT2, Proteasome 26S subunit ATPase 1, PSMC1
Rabbit Polyclonal Proteasome 19S S4/PSMC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 26S proteasome regulatory subunit 4 aa 250 to C-terminus.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Proteasome 19S S4 also known as PSMC1 is a mechanical component of the 26S proteasome an ATP-dependent protease complex responsible for protein degradation. This protein functions as an ATPase and contributes to the unfolding of ubiquitinated proteins preparing them for translocation into the 20S core particle of the proteasome for degradation. S4/PSMC1 has a molecular mass of approximately 49 kDa and is expressed ubiquitously across various cell types reflecting its essential role in cellular protein homeostasis.
PSMC1 functions as an integral part of the 19S regulatory particle of the 26S proteasome complex. This complex facilitates the recognition unfolding and translocation of polyubiquitinated proteins into the proteolytic chamber of the 20S core particle for degradation. This activity plays a significant role in regulating intracellular protein concentrations thereby controlling processes like cell cycle progression apoptosis and responses to oxidative stress. PSMC1’s activity ensures a clean cellular environment by removing damaged or nonfunctional proteins.
Functions of PSMC1 integrate into the ubiquitin-proteasome pathway and the cell cycle control pathway. In the ubiquitin-proteasome pathway PSMC1 collaborates with other ATPase subunits and regulatory proteins helping to maintain protein degradation balance and works closely with ubiquitin-conjugating enzymes responsible for tagging proteins for degradation. In the cell cycle control pathway the degradation of cyclins and cyclin-dependent kinase inhibitors by the proteasome including PSMC1 regulates cell cycle transitions and ensures proper cell division and function.
Alterations in PSMC1 function or expression can link to disorders such as cancer and neurodegenerative diseases. In cancer the dysregulation of PSMC1 can affect cellular proliferation by disrupting regulated protein degradation leading to uncontrolled cell growth. In neurodegenerative diseases such as Parkinson's impaired proteasome function including the activity of PSMC1 may lead to the accumulation of misfolded proteins contributing to neuronal cell death. Connections to other proteins like cyclins in cancer or alpha-synuclein in Parkinson's enhance the consequences of dysfunctional PSMC1 activity in these diseases.
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Immunohistochemical analysis of human testis tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in cells in seminiferous duct.
Immunohistochemical analysis of human pancreas tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in the islets of Langerhan's.
Immunohistochemical analysis of human prostate tissue labelling PSMC1 using ab223765 at 1/1000 dilution. Strong nuclear and cytoplasmic staining observed in glandular cells.
All lanes: Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) at 1/250 dilution
All lanes: EFO-21 whole cell lysate
Developed using the ECL technique.
Predicted band size: 49 kDa
All lanes: Western blot - Anti-Proteasome 19S S4/PSMC1 antibody (ab223765) at 1/250 dilution
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2: NBT-II whole cell lysate
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 49 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Proteasome 19S S4/PSMC1 (green) using ab223765 at 4 μg/ml in ICC/IF.
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