Rabbit Polyclonal Proteasome 19S S5A/ASF antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Human 26S proteasome non-ATPase regulatory subunit 4.
IgG
Rabbit
Constituents: Whole serum
Liquid
Polyclonal
WB | |
---|---|
Drosophila melanogaster | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Drosophila melanogaster | Dilution info 1/1000 | Notes - |
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Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
MCB1, PSMD4, MCB1, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, Multiubiquitin chain-binding protein, AF, ASF
Rabbit Polyclonal Proteasome 19S S5A/ASF antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 3 publications. Immunogen corresponding to Full Length Protein corresponding to Human 26S proteasome non-ATPase regulatory subunit 4.
IgG
Rabbit
Constituents: Whole serum
Liquid
Polyclonal
Whole antiserum
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The Proteasome 19S S5A also known as ASF or AH1 is a critical component of the proteasome complex. It is a regulatory particle of the 19S proteasome responsible for recognizing and binding polyubiquitin chains during protein degradation. This protein weighs approximately 48 kDa and is predominantly expressed in the cytoplasm and nucleus of eukaryotic cells. Its primary function is to facilitate the opening of the 20S core particle allowing for substrate entry into the proteasome for subsequent degradation.
The 19S S5A operates as part of a larger proteasome complex involved in maintaining protein homeostasis. It ensures efficient degradation of ubiquitinated proteins by the 26S proteasome system playing a role in cell cycle regulation signal transduction and stress responses. The S5A subunit specifically binds ubiquitin regulating the availability of substrates subject to proteolysis which is critical for the timely removal of damaged or misfolded proteins and the regulation of various intracellular processes.
The 19S S5A participates in the ubiquitin-proteasome pathway which is critical for protein turnover and quality control in cells. It interacts with proteins such as E3 ubiquitin ligases which tag substrates with ubiquitin chains for degradation. This process is also a part of the NF-kB signaling pathway where the proteasome degrades IκB proteins leading to the activation of NF-kB and modulation of immune responses.
The 19S S5A subunit has been implicated in several pathological conditions including cancer and neurodegenerative diseases. Dysregulation of the ubiquitin-proteasome system in which 19S S5A plays a role can lead to the accumulation of damaged or misfolded proteins contributing to disorders like Parkinson's and Alzheimer's disease. Abnormal activity of associated proteins such as ubiquitin ligases and other proteasome subunits often correlates with the progression of malignancies and neurodegenerative processes.
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Crude Drosophila extract. Secondary antibody: rabbit polyclonal 1:5000.
Please note: Drosophila S5a has an actual mass of 42.6 kDa, however, on the gel it runs at an apparent 54 kDa band.
All lanes: Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab18512)
Predicted band size: 40 kDa
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