AB137109

Anti-Proteasome 19S S5A/ASF antibody [EPR8936]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal Proteasome 19S S5A/ASF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

MCB1, PSMD4, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, Multiubiquitin chain-binding protein, AF, ASF

2 Images

Flow Cytometry (Intracellular) - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (AB137109)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (AB137109)

Intracellular flow cytometric analysis of permeabilized K562 cells labelling Proteasome 19S S5A/ASF with ab137109 at 1/100 dilution (red). Negative Rabbit IgG control shown in green.

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Western blot - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (AB137109)

All lanes:

Western blot - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (ab137109) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw264.7 cell lysate at 10 µg

Lane 6:

PC-12 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 40 kDa

false

Carrier free

Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8936

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Proteasome 19S S5A also known as ASF or AH1 is a critical component of the proteasome complex. It is a regulatory particle of the 19S proteasome responsible for recognizing and binding polyubiquitin chains during protein degradation. This protein weighs approximately 48 kDa and is predominantly expressed in the cytoplasm and nucleus of eukaryotic cells. Its primary function is to facilitate the opening of the 20S core particle allowing for substrate entry into the proteasome for subsequent degradation.

Biological function summary

The 19S S5A operates as part of a larger proteasome complex involved in maintaining protein homeostasis. It ensures efficient degradation of ubiquitinated proteins by the 26S proteasome system playing a role in cell cycle regulation signal transduction and stress responses. The S5A subunit specifically binds ubiquitin regulating the availability of substrates subject to proteolysis which is critical for the timely removal of damaged or misfolded proteins and the regulation of various intracellular processes.

Pathways

The 19S S5A participates in the ubiquitin-proteasome pathway which is critical for protein turnover and quality control in cells. It interacts with proteins such as E3 ubiquitin ligases which tag substrates with ubiquitin chains for degradation. This process is also a part of the NF-kB signaling pathway where the proteasome degrades IκB proteins leading to the activation of NF-kB and modulation of immune responses.

The 19S S5A subunit has been implicated in several pathological conditions including cancer and neurodegenerative diseases. Dysregulation of the ubiquitin-proteasome system in which 19S S5A plays a role can lead to the accumulation of damaged or misfolded proteins contributing to disorders like Parkinson's and Alzheimer's disease. Abnormal activity of associated proteins such as ubiquitin ligases and other proteasome subunits often correlates with the progression of malignancies and neurodegenerative processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.

See full target information PSMD4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Human cell 36:1755-1772 PubMed37336868

2023

PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Zhang,Shu Fang,Fanghao Rong,Miaomiao Jia,Yunpeng Wang,Huixian Cui,Peipei Hao

The Journal of biological chemistry 297:101275 PubMed34619150

2021

Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Min Zhang,Lei Fang,Xiaoli Yang,Ning Cao,Liyang Xu,Lihua Shi,Ying Cao

Molecular & cellular proteomics : MCP 19:1281-1302 PubMed32434922

2020

Changes in the Oligodendrocyte Progenitor Cell Proteome with Ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Alerie G de la Fuente,Rayner M L Queiroz,Tanay Ghosh,Christopher E McMurran,Juan F Cubillos,Dwight E Bergles,Denise C Fitzgerald,Clare A Jones,Kathryn S Lilley,Colin P Glover,Robin J M Franklin

Cell death & disease 10:644 PubMed31501420

2019

CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Liu,Xiao Zhang,Jiafei Lin,Yuxin Chen,Yongxia Qiao,Susu Guo,Yueyue Yang,Guoqing Zhu,Qiuhui Pan,Jiayi Wang,Fenyong Sun

Gene 702:66-74 PubMed30930224

2019

Inhibition of PSMD4 blocks the tumorigenesis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Juan Cai,Yu Cui,Min Fang,Qing Wang,Ai-Jun Zhang,Jing-Hua Kuai,Fei Pang,Xiang-Dan Cui

Proceedings of the National Academy of Sciences of 112:11229-34 PubMed26305948

2015

Structural basis of antizyme-mediated regulation of polyamine homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiang-Yi Wu,Shin-Fu Chen,Ju-Yi Hsieh,Fang Chou,Yu-Hsuan Wang,Wan-Ting Lin,Pei-Ying Lee,Yu-Jen Yu,Li-Ying Lin,Te-Sheng Lin,Chieh-Liang Lin,Guang-Yaw Liu,Shiou-Ru Tzeng,Hui-Chih Hung,Nei-Li Chan

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 41:896-905 PubMed26202105

2015

Protein Expression of Proteasome Subunits in Elderly Patients with Schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Madeline R Scott,Maria D Rubio,Vahram Haroutunian,James H Meador-Woodruff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 19S S5A/ASF antibody [EPR8936]

Flow Cytometry (Intracellular) - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (AB137109)

Western blot - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (AB137109)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise