AB248825

Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Proteasome 19S S5A/ASF antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

MCB1, PSMD4, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, Multiubiquitin chain-binding protein, AF, ASF

2 Images

Flow Cytometry (Intracellular) - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free (AB248825)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free (AB248825)

This data was developed using ab137109, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells labelling Proteasome 19S S5A/ASF with ab137109 at 1/100 dilution (red). Negative Rabbit IgG control shown in green.

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Western blot - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free (AB248825)

This data was developed using ab137109, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] (<a href='/en-us/products/primary-antibodies/proteasome-19s-s5a-asf-antibody-epr8936-ab137109'>ab137109</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw264.7 cell lysate at 10 µg

Lane 6:

PC-12 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 40 kDa

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Unconjugated

Anti-Proteasome 19S S5A/ASF antibody [EPR8936]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8936

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab248825 is the carrier-free version of ab137109.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Proteasome 19S S5A also known as ASF or AH1 is a critical component of the proteasome complex. It is a regulatory particle of the 19S proteasome responsible for recognizing and binding polyubiquitin chains during protein degradation. This protein weighs approximately 48 kDa and is predominantly expressed in the cytoplasm and nucleus of eukaryotic cells. Its primary function is to facilitate the opening of the 20S core particle allowing for substrate entry into the proteasome for subsequent degradation.

Biological function summary

The 19S S5A operates as part of a larger proteasome complex involved in maintaining protein homeostasis. It ensures efficient degradation of ubiquitinated proteins by the 26S proteasome system playing a role in cell cycle regulation signal transduction and stress responses. The S5A subunit specifically binds ubiquitin regulating the availability of substrates subject to proteolysis which is critical for the timely removal of damaged or misfolded proteins and the regulation of various intracellular processes.

Pathways

The 19S S5A participates in the ubiquitin-proteasome pathway which is critical for protein turnover and quality control in cells. It interacts with proteins such as E3 ubiquitin ligases which tag substrates with ubiquitin chains for degradation. This process is also a part of the NF-kB signaling pathway where the proteasome degrades IκB proteins leading to the activation of NF-kB and modulation of immune responses.

The 19S S5A subunit has been implicated in several pathological conditions including cancer and neurodegenerative diseases. Dysregulation of the ubiquitin-proteasome system in which 19S S5A plays a role can lead to the accumulation of damaged or misfolded proteins contributing to disorders like Parkinson's and Alzheimer's disease. Abnormal activity of associated proteins such as ubiquitin ligases and other proteasome subunits often correlates with the progression of malignancies and neurodegenerative processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.

See full target information PSMD4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free (AB248825)

Western blot - Anti-Proteasome 19S S5A/ASF antibody [EPR8936] - BSA and Azide free (AB248825)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise