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Mouse Monoclonal PSA1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Full Length Protein corresponding to Human PSMA1.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674), expandable thumbnail
  • Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (AB22674), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Human PSMA1. The exact immunogen used to generate this antibody is proprietary information. Database link P25786

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IF
Human
Not recommended
Tested
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Does not immunoprecipitate intact proteasomes from HeLa cell preparations.

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

Targets

PSMA2, PSMA3, PSMA5, PSMA6, PSMA7

Alternative names

Proteasome subunit alpha type-1, 30 kDa prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, PROS-30, PSC2, PROS30, NU, PSMA1, HC2

Recommended products

Mouse Monoclonal PSA1 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Full Length Protein corresponding to Human PSMA1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Human PSMA1. The exact immunogen used to generate this antibody is proprietary information. Database link P25786
Clone number

MCP231

Purification technique

Affinity purification Protein G

Epitope

Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Dilute antibody to working dilution in PBS, pH 7.2 - 7.4. Store at 4°C and use within 1 month. Store unopened vial at -20°C for long term storage. Avoid freeze/thaw cycles.

This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Proteasome 20S alpha subunits (also known as proteasome marker proscrate) are parts of the core structure of the 20S proteasome. This target features the six alpha subunits: alpha 1 alpha 2 alpha 3 alpha 5 alpha 6 and alpha 7. These alpha subunits collectively form the gate of the proteolytic chamber regulating substrate entry. The mass of the entire 20S proteasome complex is around 700 kDa. This target is ubiquitously expressed in eukaryotic cells playing a critical role in protein degradation.

Biological function summary

The 20S alpha subunit functions as part of the larger 26S proteasome complex which includes other components such as 19S regulatory particles. It facilitates protein turnover by degrading misfolded damaged or unneeded proteins into peptides. The 20S proteasome subunits' activity is essential in maintaining cellular homeostasis and controlling the concentration of specific proteins. They contribute to processes like cell cycle regulation and antigen presentation.

Pathways

The 20S proteasome involves itself directly in the ubiquitin-proteasome pathway a fundamental pathway for protein degradation. It is an important player in the immune response pathway aiding in processing peptides for major histocompatibility complex (MHC) class I antigen presentation. This function connects the 20S proteasome to other proteins such as ubiquitin and molecules involved in antigen processing.

Associated diseases and disorders

Dysregulation of 20S proteasome activity associates with conditions such as cancer and neurodegenerative diseases. In cancer increased proteasome activity supports rapid cell division by degrading cell cycle inhibitors. Furthermore research links impaired proteasome function in neurodegenerative disorders such as Alzheimer's disease where inefficient protein degradation leads to the accumulation of toxic protein aggregates. The target connects to proteins like amyloid precursor protein in Alzheimer's emphasizing the critical role of proteasome subunits in disease pathogenesis.

Product promise

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Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

    ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.


    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674), expandable thumbnail

    Flow Cytometry - Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)

    Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


    This image was generated using the ascites version of the product.

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Product protocols

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