AB109525

Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]

Name: Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) | Abcam
Brand: Abcam Limited
SKU: AB109525
Price: 330 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal Proteasome 20S alpha 2/HC3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HC3, PSC3, PSMA2, Proteasome subunit alpha type-2, Macropain subunit C3, Multicatalytic endopeptidase complex subunit C3, Proteasome component C3, Proteasome subunit alpha-2, alpha-2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

ab109525 at 1/50 dilution staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

ab109525 at a 1/250 dilution staining Human Proteasome 20S alpha 2/HC3 in brain tissue using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded).

Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

All lanes:

Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

JAR cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5453

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S alpha 2 also known as HC3 forms part of the proteasome complex responsible for protein degradation. This protein has a molecular mass of approximately 25 kDa and functions as a structural component of the 20S core particle within the proteasome. The 20S alpha 2 subunit is highly conserved and expressed in various tissues contributing to cellular protein homeostasis through the removal of damaged or misfolded proteins.

Biological function summary

The 20S alpha 2 subunit integrates into the larger 26S proteasome complex working alongside other subunits to regulate protein turnover. This complex controls the degradation of ubiquitin-tagged proteins impacting diverse cellular processes like cell cycle progression and stress response. Through its role in the proteasome HC3 maintains protein quality control within the cell and supports proper cellular function.

Pathways

Proteasome 20S alpha 2 interacts within the ubiquitin-proteasome pathway and plays a central role in protein degradation and turnover. It associates with related proteins such as other members of the proteolytic complex and interacts with ubiquitin ligases to efficiently process and degrade proteins tagged for destruction. By facilitating these pathways it is critical for cellular regulation and response to changes.

Proteasome 20S alpha 2 has associations with several pathological conditions including neurodegenerative diseases and cancer. The malfunction or altered expression of this subunit can impact protein degradation contributing to the accumulation of malfunctioning proteins linked to these diseases. It interacts with proteins like p53 in cancer where proteasomal regulation can affect tumorigenesis and with neurofibrillary tangles in Alzheimer's disease affecting neuronal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 189:1363-1374 PubMed31026418

2019

Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Min Ni,Xiaojuan Chao,Joshua Kaseff,Fengyan Deng,Shaogui Wang,Ying-Hong Shi,Tiangang Li,Wen-Xing Ding,Hartmut Jaeschke

Molecular cell 71:1079-1091.e9 PubMed30146318

2018

Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Brigitta Alber,Eric Raphael Paquet,Martina Biserni,Felix Naef,David Michael Suter

Oncotarget 7:17681-98 PubMed26769846

2016

Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaogui Wang,Hong-Min Ni,Kenneth Dorko,Sean C Kumer,Timothy M Schmitt,Atta Nawabi,Masaaki Komatsu,Heqing Huang,Wen-Xing Ding

Cell death & disease 5:e1066 PubMed24556681

2014

TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain.

Applications

Species

Mouse

A Pla,M Pascual,J Renau-Piqueras,C Guerri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (AB109525)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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