AB109502

Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Proteasome 20S alpha 2/HC3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HC3, PSC3, PSMA2, Proteasome subunit alpha type-2, Macropain subunit C3, Multicatalytic endopeptidase complex subunit C3, Proteasome component C3, Proteasome subunit alpha-2, alpha-2

2 Images

Flow Cytometry (Intracellular) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (AB109502)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (AB109502)

Intracellular flow cytometric analysis of permeabilized HeLa cells using ab109502 at 1/10 dilution (red) and a control rabbit IgG (green).

Unknown

Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (AB109502)

All lanes:

Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (ab109502) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

JAR cell lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Carrier free

Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5454

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S alpha 2 also known as HC3 forms part of the proteasome complex responsible for protein degradation. This protein has a molecular mass of approximately 25 kDa and functions as a structural component of the 20S core particle within the proteasome. The 20S alpha 2 subunit is highly conserved and expressed in various tissues contributing to cellular protein homeostasis through the removal of damaged or misfolded proteins.

Biological function summary

The 20S alpha 2 subunit integrates into the larger 26S proteasome complex working alongside other subunits to regulate protein turnover. This complex controls the degradation of ubiquitin-tagged proteins impacting diverse cellular processes like cell cycle progression and stress response. Through its role in the proteasome HC3 maintains protein quality control within the cell and supports proper cellular function.

Pathways

Proteasome 20S alpha 2 interacts within the ubiquitin-proteasome pathway and plays a central role in protein degradation and turnover. It associates with related proteins such as other members of the proteolytic complex and interacts with ubiquitin ligases to efficiently process and degrade proteins tagged for destruction. By facilitating these pathways it is critical for cellular regulation and response to changes.

Proteasome 20S alpha 2 has associations with several pathological conditions including neurodegenerative diseases and cancer. The malfunction or altered expression of this subunit can impact protein degradation contributing to the accumulation of malfunctioning proteins linked to these diseases. It interacts with proteins like p53 in cancer where proteasomal regulation can affect tumorigenesis and with neurofibrillary tangles in Alzheimer's disease affecting neuronal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:9411276 PubMed36406766

2022

Inhibition of MicroRNA-182/183 Cluster Ameliorates Schizophrenia by Activating the Axon Guidance Pathway and Upregulating DCC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Wang,Lin Su,Tong Wu,Lei Sun,Zhenghai Sun,Yuchen Wang,Ping Li,Guangcheng Cui

Cell death and differentiation 29:1513-1527 PubMed35105963

2022

EBV infection-induced GPX4 promotes chemoresistance and tumor progression in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yuan,Shibing Li,Qiuyan Chen,Tianliang Xia,Donghua Luo,Liangji Li,Sailan Liu,Shanshan Guo,Liting Liu,Chaochao Du,Guodong Jia,Xiaoyun Li,Zijian Lu,Zhenchong Yang,Huanliang Liu,Haiqiang Mai,Linquan Tang

Journal of Cancer 12:3325-3334 PubMed33976742

2021

The Yunnan national medicine Maytenus compound inhibits the proliferation of hepatocellular carcinoma (HCC) by suppressing the activation of the EGFR-PI3K-AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Tao Zhao,Liu-Xin Han,Lin Liu,Bao-Zhen Zeng,Yi Zhang,Liu-Fang Zhao,Hong-Yan Hu,Jia-Wei Xia,Yi-Ze Li,Xu-Dong Xiang,Xiao-Lin Lin,Di Lu,Gao-Feng Li

OncoTargets and therapy 13:12015-12025 PubMed33244243

2020

Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Niu,Liang Shan,Han Gao,Congcong Zhang,Zijun Qian,Zhixian Wang,Xin Xu,Xiao Zhang,Jiayi Wang,Lifang Ma,Liyun Chen,Yongchun Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454]

Flow Cytometry (Intracellular) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (AB109502)

Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5454] (AB109502)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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