Rabbit Polyclonal PSA5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Proteasome subunit alpha type-5 aa 100-150.
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- Veterinary immunology and immunopathology 226:1100692020The IFN-γ-induced immunoproteasome is suppressed in highly pathogenic porcine reproductive and respiratory syndrome virus-infected alveolar macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiang Liu et. al.PubMed 32535163
- The EMBO journal 38:e992662019Proteasomal degradation within endocytic organelles mediates antigen cross-presentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDebrup Sengupta et. al.PubMed 31271236
- Scientific reports 8:96462018Accumulation of poly(A) RNA in nuclear granules enriched in Sam68 in motor neurons from the SMNΔ7 mouse model of SMA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Oriol Narcís et. al.PubMed 29941967
- Experimental and therapeutic medicine 15:4298-43082018Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunhui Wang et. al.PubMed 29849774
- Oncotarget 7:20532-482016Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects.Applications:WBReactive species:Unspecified reactive speciesFederica Mori,Maria Ferraiuolo,Raffaela Santoro,Andrea Sacconi,Frauke Goeman,Matteo Pallocca,Claudio Pulito,Etleva Korita,Maurizio Fanciulli,Paola Muti,Giovanni Blandino,Sabrina StranoPubMed 26967561
- PloS one 8:e524082013Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNβ in mouse pancreatic β-cells.
- Virology journal 8:3652011Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16.
- Molecular & cellular proteomics : MCP 9:2034-472010Deletion of immunoproteasome subunits imprints on the transcriptome and has a broad impact on peptides presented by major histocompatibility complex I molecules.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Proteasome subunit alpha type-5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P28066
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Alternative names
Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome subunit alpha-5, Proteasome zeta chain, alpha-5, PSMA5
Recommended products
Rabbit Polyclonal PSA5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Proteasome subunit alpha type-5 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Proteasome subunit alpha type-5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P28066
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proteasome 20S alpha 5 also known as PSMA5 is a subunit of the 20S proteasome core particle playing a role in protein degradation. This subunit weighs approximately 26 kDa and is expressed broadly across various tissues. PSMA5 forms part of the alpha ring of the proteasome and contributes to the gating mechanism controlling the entry of substrates into the catalytic core.
- Biological function summary
PSMA5 functions within the multicatalytic proteinase complex known as the 20S proteasome. The proteasome is critical for degrading ubiquitinated proteins maintaining cellular protein homeostasis and regulating protein levels in the cell. By breaking down faulty or misfolded proteins and proteins involved in cell cycle regulation PSMA5 influences the turnover of proteins and ensures cellular function. PSMA5 is essential for cellular processes such as transcription cell cycle progression and apoptosis.
- Pathways
PSMA5 has significant roles in both the ubiquitin-proteasome pathway and the NF-kB signaling pathway. In the ubiquitin-proteasome pathway PSMA5 helps degrade proteins tagged for destruction by ubiquitin involving components like ubiquitin-conjugating enzymes. In the NF-kB pathway the proteasome degrades inhibitors of NF-kB enabling the activation of this transcription factor and therefore facilitating gene expression related to immune and inflammatory responses.
- Associated diseases and disorders
PSMA5 has associations with cancer and neurodegenerative diseases. In cancer the overactivity of proteasome including PSMA5 can prevent the accumulation of pro-apoptotic factors promoting cell survival and tumor growth. Bortezomib a proteasome inhibitor targets this process to induce tumor cell death. In neurodegenerative diseases like Alzheimer's alterations in proteasome function may lead to the accumulation of toxic protein aggregates. Here PSMA5 interacts with proteins related to these conditions impacting disease progression and response to therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
All lanes: Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Lane 1: HeLa cell extract.
Lane 2: HeLa cell extract with blocking peptide.
Predicted band size: 26 kDa
Observed band size: 26.5 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Immunofluorescence of HeLa cells using ab11437.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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