AB189855

Anti-Proteasome 20S alpha 5/PSMA5 antibody

Name: Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab189855) | Abcam
Brand: Abcam Limited
SKU: AB189855

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(2 Publications)

Rabbit Polyclonal PSA5 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Proteasome subunit alpha type-5.

View Alternative Names

Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome subunit alpha-5, Proteasome zeta chain, alpha-5, PSMA5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (AB189855)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (AB189855)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab189855. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (AB189855)

All lanes:

Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab189855) at 1/500 dilution

Lane 1:

MCF7 cell extract

Lane 2:

293T cell extract

Lane 3:

HepG2 cell extract

Lane 4:

U-251MG cell extract

Lane 5:

BxPC3 cell extract

Lane 6:

Mouse liver extract

Lane 7:

Mouse thymus extract

Lane 8:

Mouse heart extract

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Proteasome subunit alpha type-5.

P28066

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S alpha 5 also known as PSMA5 is a subunit of the 20S proteasome core particle playing a role in protein degradation. This subunit weighs approximately 26 kDa and is expressed broadly across various tissues. PSMA5 forms part of the alpha ring of the proteasome and contributes to the gating mechanism controlling the entry of substrates into the catalytic core.

Biological function summary

PSMA5 functions within the multicatalytic proteinase complex known as the 20S proteasome. The proteasome is critical for degrading ubiquitinated proteins maintaining cellular protein homeostasis and regulating protein levels in the cell. By breaking down faulty or misfolded proteins and proteins involved in cell cycle regulation PSMA5 influences the turnover of proteins and ensures cellular function. PSMA5 is essential for cellular processes such as transcription cell cycle progression and apoptosis.

Pathways

PSMA5 has significant roles in both the ubiquitin-proteasome pathway and the NF-kB signaling pathway. In the ubiquitin-proteasome pathway PSMA5 helps degrade proteins tagged for destruction by ubiquitin involving components like ubiquitin-conjugating enzymes. In the NF-kB pathway the proteasome degrades inhibitors of NF-kB enabling the activation of this transcription factor and therefore facilitating gene expression related to immune and inflammatory responses.

PSMA5 has associations with cancer and neurodegenerative diseases. In cancer the overactivity of proteasome including PSMA5 can prevent the accumulation of pro-apoptotic factors promoting cell survival and tumor growth. Bortezomib a proteasome inhibitor targets this process to induce tumor cell death. In neurodegenerative diseases like Alzheimer's alterations in proteasome function may lead to the accumulation of toxic protein aggregates. Here PSMA5 interacts with proteins related to these conditions impacting disease progression and response to therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information Proteasome subunit alpha type-5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 26:558-568.e9 PubMed32109376

2020

Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S Morrow,Tiaira J Porter,Nan Xu,Zachary P Arndt,Kayla Ako-Asare,Helen J Heo,Elizabeth A N Thompson,Darcie L Moore

International journal of molecular sciences 20: PubMed31262043

2019

IL-13 Impairs Tight Junctions in Airway Epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Schmidt,Peter Braubach,Carolin Schilpp,Robin Lochbaum,Kathrin Neuland,Kristin Thompson,Danny Jonigk,Manfred Frick,Paul Dietl,Oliver H Wittekindt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 20S alpha 5/PSMA5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (AB189855)

Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (AB189855)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise