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AB22673

Anti-Proteasome 20S alpha + beta antibody

5

(7 Reviews)

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(43 Publications)

Anti-Proteasome 20S alpha + beta antibody (ab22673) is a rabbit polyclonal antibody detecting Proteasome 20S alpha + beta in Western Blot, IP, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat, Saccharomyces cerevisiae.

- Over 30 publications
- Trusted since 2005

View Alternative Names

HSPC, PSMA7, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7, Proteasome subunit alpha-4, alpha-4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (AB22673)
  • ICC/IF

Project

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (AB22673)

ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.

This image is courtesy of an anonymous abreview.

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (AB22673)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha + beta antibody (AB22673)

Immunofluorescence analysis of HeLa cells, staining Proteasome 20S alpha + beta with ab22673.

Cells were fixed with formaldehyde and permeabilized using 0.1% Triton X-100. Samples were incubated with primary antibody (1/1000) fr 1 hour at room temperature. An AlexaFluor®555-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

Image from Anderson DD et al., J Biol Chem. 2012 Feb 10;287(7):4790-9. Epub 2011 Dec 21. Fig 5.; doi: 10.1074/jbc.M111.302174; February 10, 2012 The Journal of Biological Chemistry, 287, 4790-4799.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Saccharomyces cerevisiae, Rat, Mouse

Applications

ICC/IF, IP, IHC-Fr, WB

applications

Immunogen

Cell preparation containing PSMA1 protein. The exact immunogen used to generate this antibody is proprietary information.

P25786

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Proteasome 20S alpha + beta antibody (ab22673) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat, Saccharomyces cerevisiae samples.

Trusted by the scientific community
Anti-Proteasome 20S alpha + beta (ab22673) was first used in a scientific publication in 2005 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Proteasome 20S alpha + beta (ab22673) has over 5 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.065% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteasome 20S alpha + beta sometimes referred to simply as the 20S proteasome consists of the catalytic core of the larger 26S proteasome complex. This core degrades ubiquitinated proteins in eukaryotic cells. The 20S proteasome has a barrel-like structure composed of 28 subunits arranged in four stacked rings. Each alpha and beta ring contains seven subunits with masses approximately ranging from 20 to 31 kDa. The proteasome 20S alpha + beta is expressed in the cytoplasm and nucleus of cells important for maintaining protein homeostasis by removing damaged or misfolded proteins as well as short-lived regulatory proteins.
Biological function summary

The proteasome 20S alpha + beta plays a central role in protein degradation ensuring cellular function by regulating the concentration of specific proteins. It is an important component of the ubiquitin-proteasome system (UPS) a complex important for protein turnover. The 20S core particle combines with regulatory particles to form the 26S proteasome which recognizes and unfolds ubiquitinated proteins before translocation into the core for degradation. By controlling the half-life of critical proteins the proteasome influences cellular processes such as the cell cycle apoptosis and immune responses.

Pathways

The ubiquitin-proteasome system including the proteasome 20S alpha + beta is integral to protein homeostasis and the cell cycle control pathway. It removes cyclins key regulators of cell cycle progression illustrating its regulatory function. Another significant pathway is the NF-kB signaling pathway where the proteasome degrades inhibitors to activate NF-kB a transcription factor involved in inflammation and immune responses. These pathways highlight the proteasome's relationship with proteins like cyclins and IkBa emphasizing its role in cellular regulation.

Proteasome dysfunction links to neurodegenerative diseases such as Parkinson's disease and certain cancers. Aberrations in proteasomal activity can result in the accumulation of toxic protein aggregates contributing to neurodegenerative processes. In cancers altered regulation of the proteasome may promote cell survival and proliferation. The proteasome is connected to proteins like alpha-synuclein in Parkinson’s disease and p53 a tumor suppressor in cancer highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
See full target information PSMA7

Additional targets

PSMA1,Proteasome subunit alpha type-5,PSMB1,PSMB6,PSMB5
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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Nature 641:202-210 PubMed40140567

2025

STING agonist-based ER-targeting molecules boost antigen cross-presentation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiafeng Wang,Zhangping Huang,Lixiao Xing,Liru Shang,Juan Jiang,Caiguanxi Deng,Wei Yu,Lin Peng,Hao Yang,Xiaohong Zheng,Xinmin Liu,Haolan Yang,Yixin Chen,Yongyong Li,Jing Liu,Xi Xie,Wei Xu,Xiaojun Xia,Zezhong Liu,Wanli Liu,Shibo Jiang,Yingyue Zeng,Lu Lu,Ji Wang

Biology 13: PubMed39452152

2024

Short-Term Proteasome Inhibition: Assessment of the Effects of Carfilzomib and Bortezomib on Cardiac Function, Arterial Stiffness, and Vascular Reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Callan D Wesley,Annarita Sansonetti,Cedric H G Neutel,Dustin N Krüger,Guido R Y De Meyer,Wim Martinet,Pieter-Jan Guns

Autophagy 21:583-597 PubMed39385699

2024

Empagliflozin protects the kidney by reducing toxic ALB (albumin) exposure and preventing autophagic stagnation in proximal tubules.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Matsui,Takeshi Yamamoto,Yoshitsugu Takabatake,Atsushi Takahashi,Tomoko Namba-Hamano,Jun Matsuda,Satoshi Minami,Shinsuke Sakai,Hiroaki Yonishi,Jun Nakamura,Shihomi Maeda,Ayumi Matsumoto,Isao Matsui,Motoko Yanagita,Yoshitaka Isaka

Nature communications 15:7891 PubMed39256395

2024

A secreted fungal laccase targets the receptor kinase OsSRF3 to inhibit OsBAK1-OsSRF3-mediated immunity in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhang Duan,Zhaoyun Wang,Yuan Fang,Zhangxin Pei,Hong Hu,Qiutao Xu,Hao Liu,Xiaolin Chen,Chaoxi Luo,Junbin Huang,Lu Zheng,Xiaoyang Chen

Aging 15:3295-3330 PubMed37179123

2023

Proteostatic modulation in brain aging without associated Alzheimer's disease-and age-related neuropathological changes.

Applications

Unspecified application

Species

Unspecified reactive species

Pol Andrés-Benito,Ignacio Íñigo-Marco,Marta Brullas,Margarita Carmona,José Antonio Del Rio,Joaquín Fernández-Irigoyen,Enrique Santamaría,Mónica Povedano,Isidro Ferrer

iScience 26:106232 PubMed36876137

2023

COP I and II dependent trafficking controls ER-associated degradation in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Navit Ogen-Shtern,Chieh Chang,Haddas Saad,Niv Mazkereth,Chaitanya Patel,Marina Shenkman,Gerardo Z Lederkremer

Science advances 7:eabi6794 PubMed34797715

2021

Deubiquitination of proteasome subunits by OTULIN regulates type I IFN production.

Applications

Unspecified application

Species

Unspecified reactive species

Panfeng Tao,Shihao Wang,Seza Ozen,Pui Y Lee,Jiahui Zhang,Jun Wang,Huan Han,Zhaohui Yang,Ran Fang,Wanxia Li Tsai,Huanming Yang,Erdal Sag,Rezan Topaloglu,Ivona Aksentijevich,Xiaomin Yu,Qing Zhou

Molecules (Basel, Switzerland) 26: PubMed34834058

2021

Plasma Exosomes of Patients with Breast and Ovarian Tumors Contain an Inactive 20S Proteasome.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Yunusova,Elena Kolegova,Elena Sereda,Larisa Kolomiets,Alisa Villert,Marina Patysheva,Irina Rekeda,Alina Grigor'eva,Natalia Tarabanovskaya,Irina Kondakova,Svetlana Tamkovich

The Journal of biological chemistry 297:101275 PubMed34619150

2021

Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Min Zhang,Lei Fang,Xiaoli Yang,Ning Cao,Liyang Xu,Lihua Shi,Ying Cao

Frontiers in cell and developmental biology 9:650817 PubMed34055780

2021

Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Ibañez-Vega,Felipe Del Valle,Juan José Sáez,Fanny Guzman,Jheimmy Diaz,Andrea Soza,María Isabel Yuseff
View all publications
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