AB150366

Anti-Proteasome 20S beta 6 antibody [EPR9685]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PSB6 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

LMPY, Y, PSMB6, Proteasome subunit beta type-6, Macropain delta chain, Multicatalytic endopeptidase complex delta chain, Proteasome delta chain, Proteasome subunit Y, Proteasome subunit beta-1, beta-1

2 Images

Flow Cytometry (Intracellular) - Anti-Proteasome 20S beta 6 antibody [EPR9685] (AB150366)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Proteasome 20S beta 6 antibody [EPR9685] (AB150366)

Intracellular flow cytometric analysis of permeabilized HeLa cells labelling Proteasome 20S beta 6 using ab150366 (red) or a rabbit IgG control (green).

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Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9685] (AB150366)

All lanes:

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9685] (ab150366) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9685

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S beta 6 also known as PSMB6 is a subunit of the proteasome complex. It plays a significant role in the protein degradation process. Its molecular weight is around 25 kDa. The subunit gets expressed in various cell types throughout the body ensuring that proteins are effectively broken down into peptides. This is essential for maintaining protein homeostasis and recycling faulty proteins.

Biological function summary

The proteasome 20S beta 6 subunit forms part of the 20S core complex an important component of the proteasome. The proteasome operates as the main proteolytic machinery in both nucleus and cytoplasm. The 20S core complex pairs with the regulatory 19S cap to form the 26S proteasome. This assembly degrades ubiquitin-tagged proteins which regulates cell cycle progression apoptosis and DNA repair contributing to cellular function and health.

Pathways

Proteasome 20S beta 6 acts within the ubiquitin-proteasome pathway. This pathway regulates protein turnover affecting critical cellular processes such as cell cycle apoptosis and the immune response. The pathway also links with other proteins such as ubiquitin and E1 E2 and E3 ligases which modify proteins with ubiquitin chains to signal for their degradation.

Proteasome 20S beta 6 may relate to cancer and neurodegenerative diseases like Alzheimer's. The abnormal regulation of the proteasome pathway can lead to the accumulation of undegraded proteins contributing to these diseases. PSMB6 interacts with proteins such as p53 a tumor suppressor protein highlighting its relevance to cell cycle control and cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.

See full target information PSMB6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 20:20251206 PubMed40918165

2025

Peptide CCAT1-70aa promotes hepatocellular carcinoma proliferation and invasion via the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjian Wu,Ruifeng Liang,Guoman Liu,Quan Fang,Zuoming Xu,Wenchuan Li,Chuan Tan,Jian Pu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Proteasome 20S beta 6 antibody [EPR9685]

Flow Cytometry (Intracellular) - Anti-Proteasome 20S beta 6 antibody [EPR9685] (AB150366)

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9685] (AB150366)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise