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AB248944

Anti-Proteasome 20S beta 6 antibody [EPR9685] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PSB6 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LMPY, Y, PSMB6, Proteasome subunit beta type-6, Macropain delta chain, Multicatalytic endopeptidase complex delta chain, Proteasome delta chain, Proteasome subunit Y, Proteasome subunit beta-1, beta-1

2 Images
Flow Cytometry (Intracellular) - Anti-Proteasome 20S beta 6 antibody [EPR9685] - BSA and Azide free (AB248944)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Proteasome 20S beta 6 antibody [EPR9685] - BSA and Azide free (AB248944)

This data was developed using ab150366, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labelling Proteasome 20S beta 6 using ab150366 (red) or a rabbit IgG control (green).

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9685] - BSA and Azide free (AB248944)
  • WB

Unknown

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9685] - BSA and Azide free (AB248944)

This data was developed using ab150366, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Proteasome 20S beta 6 antibody [EPR9685] (<a href='/en-us/products/primary-antibodies/proteasome-20s-beta-6-antibody-epr9685-ab150366'>ab150366</a>) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

false

  • Unconjugated

    Anti-Proteasome 20S beta 6 antibody [EPR9685]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9685

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

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Proteasome Activity Assay Kit

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (AB166628)

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Primary Antibodies

AB3329

Anti-Proteasome 20S LMP7 antibody

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Primary Antibodies

AB22673

Anti-Proteasome 20S alpha + beta antibody

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Primary Antibodies

AB171969

Anti-TBP-1 antibody [EPR12152] - N-terminal

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBP-1 antibody [EPR12152] - N-terminal (AB171969)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248944 is the carrier-free version of ab150366.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome 20S beta 6 also known as PSMB6 is a subunit of the proteasome complex. It plays a significant role in the protein degradation process. Its molecular weight is around 25 kDa. The subunit gets expressed in various cell types throughout the body ensuring that proteins are effectively broken down into peptides. This is essential for maintaining protein homeostasis and recycling faulty proteins.
Biological function summary

The proteasome 20S beta 6 subunit forms part of the 20S core complex an important component of the proteasome. The proteasome operates as the main proteolytic machinery in both nucleus and cytoplasm. The 20S core complex pairs with the regulatory 19S cap to form the 26S proteasome. This assembly degrades ubiquitin-tagged proteins which regulates cell cycle progression apoptosis and DNA repair contributing to cellular function and health.

Pathways

Proteasome 20S beta 6 acts within the ubiquitin-proteasome pathway. This pathway regulates protein turnover affecting critical cellular processes such as cell cycle apoptosis and the immune response. The pathway also links with other proteins such as ubiquitin and E1 E2 and E3 ligases which modify proteins with ubiquitin chains to signal for their degradation.

Proteasome 20S beta 6 may relate to cancer and neurodegenerative diseases like Alzheimer's. The abnormal regulation of the proteasome pathway can lead to the accumulation of undegraded proteins contributing to these diseases. PSMB6 interacts with proteins such as p53 a tumor suppressor protein highlighting its relevance to cell cycle control and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the peptides bond hydrolysis occurs directly after acidic residues.
See full target information PSMB6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com