AB140499

Anti-Proteasome 20S C2/HC2 antibody

Name: Anti-Proteasome 20S C2/HC2 antibody (ab140499) | Abcam
Brand: Abcam Limited
SKU: AB140499
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal PSA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PSMA1 aa 200 to C-terminus.

View Alternative Names

HC2, NU, PROS30, PSC2, PSMA1, Proteasome subunit alpha type-1, 30 kDa prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, Proteasome subunit alpha-6, PROS-30, alpha-6

2 Images

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Immunoprecipitation - Anti-Proteasome 20S C2/HC2 antibody (AB140499)

ab140499 at 1 μg/ml detecting Proteasome 20S C2/HC2 in 293T whole cell lysate by WB following IP.

Lane 1 : ab140499 at 6μg/mg of lysate.
Lane 2 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Proteasome 20S C2/HC2 antibody (ab140499)

Predicted band size: 30 kDa

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Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB140499)

All lanes:

Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab140499) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 30 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PSMA1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25786

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab140499 was affinity purified using an epitope specific to Proteasome 20S C2/HC2 immobilized on a solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Proteasome 20S C2/HC2 also known as the proteasome subunit beta type-1 represents an important component of the proteasome complex. This protein performs proteolytic functions degrading ubiquitinated proteins into peptides. The proteasome 20S C2/HC2 has a molecular mass of approximately 23 kDa and is expressed in various tissues throughout the body including muscle and nerve tissues. It forms part of the 20S core particle of the proteasome complex and contributes to protein homeostasis.

Biological function summary

Proteasome 20S C2/HC2 plays a significant role in maintaining protein quality by participating in proteolytic degradation. It forms part of the larger 26S proteasome complex which assembles with regulatory particles for substrate recognition. This protein is essential for the breakdown and removal of oxidized or misfolded proteins thereby preventing the accumulation of potentially toxic aggregates within the cell. It ensures cellular regulation by processing proteins that control various aspects of cell cycle signaling and differentiation.

Pathways

The activity of the Proteasome 20S C2/HC2 integrates directly into the ubiquitin-proteasome pathway. This pathway regulates protein turnover and plays a vital role in cellular processes such as the cell cycle and apoptosis. The association with proteins such as ubiquitin and E3 ligases is critical for the marking of substrate proteins for degradation. This protein is also linked to the NF-kB signaling pathway interacting with proteins that modulate immune and inflammatory responses demonstrating its influence on broader regulatory networks.

Proteasome 20S C2/HC2 disruption has been identified in various pathological conditions. Abnormal function or expression levels relate to neurodegenerative disorders like Parkinson’s disease where inefficient protein degradation leads to protein aggregation. This protein also connects to tumor progression in cancers where alterations in the ubiquitin-proteasome system affect cell cycle regulation and apoptosis. The protein's interaction with tumor suppressors and oncogenes highlights its significance in cellular growth abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular systems biology 20:403-427 PubMed38287148

2024

Systematic identification of 20S proteasome substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Pepelnjak,Rivkah Rogawski,Galina Arkind,Yegor Leushkin,Irit Fainer,Gili Ben-Nissan,Paola Picotti,Michal Sharon

Nature communications 14:3126 PubMed37253751

2023

Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family.

Applications

Unspecified application

Species

Unspecified reactive species

Fanindra Kumar Deshmukh,Gili Ben-Nissan,Maya A Olshina,Maria G Füzesi-Levi,Caley Polkinghorn,Galina Arkind,Yegor Leushkin,Irit Fainer,Sarel J Fleishman,Dan Tawfik,Michal Sharon

Nature communications 13:6228 PubMed36266274

2022

A BDNF-TrkB autocrine loop enhances senescent cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Anerillas,Allison B Herman,Rachel Munk,Amanda Garrido,Kwan-Wood Gabriel Lam,Matthew J Payea,Martina Rossi,Dimitrios Tsitsipatis,Jennifer L Martindale,Yulan Piao,Krystyna Mazan-Mamczarz,Jinshui Fan,Chang-Yi Cui,Supriyo De,Kotb Abdelmohsen,Rafael de Cabo,Myriam Gorospe

EMBO reports 23:e54755 PubMed35642585

2022

Malaria parasites release vesicle subpopulations with signatures of different destinations.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Abou Karam,Irit Rosenhek-Goldian,Tamar Ziv,Hila Ben Ami Pilo,Ido Azuri,Anna Rivkin,Edo Kiper,Ron Rotkopf,Sidney R Cohen,Ana Claudia Torrecilhas,Ori Avinoam,Alicia Rojas,Mattia I Morandi,Neta Regev-Rudzki

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki

Antioxidants & redox signaling 32:636-655 PubMed31903784

2020

Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Maya A Olshina,Galina Arkind,Fanindra Kumar Deshmukh,Irit Fainer,Mark Taranavsky,Daniel Hayat,Shifra Ben-Dor,Gili Ben-Nissan,Michal Sharon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Proteasome 20S C2/HC2 antibody

Immunoprecipitation - Anti-Proteasome 20S C2/HC2 antibody (AB140499)

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB140499)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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