AB3325

Anti-Proteasome 20S C2/HC2 antibody

Name: Anti-Proteasome 20S C2/HC2 antibody (ab3325) | Abcam
Brand: Abcam Limited
SKU: AB3325
Price: 585 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(39 Publications)

Rabbit Polyclonal PSA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Chinese hamster, Human, Mouse, Rat, Hamster, Dog samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human PSMA1 aa 200 to C-terminus.

View Alternative Names

HC2, NU, PROS30, PSC2, PSMA1, Proteasome subunit alpha type-1, 30 kDa prosomal protein, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, Proteasome subunit alpha-6, PROS-30, alpha-6

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

Immunofluorescence analysis of 70% confluent log phase MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Proteasome 20S C2/HC2 (green) with ab3325 at 2 μg/mL. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3325 in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

Supplier Data

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

All lanes:

Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 2 µg/mL

Lane 1:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Lane 5:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP cpnjugate at 0.4 µg/mL

Predicted band size: 30 kDa

true

Unknown

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

All lanes:

Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 3 µg/mL

All lanes:

CHO (Chinese hamster ovary cell line) whole cell lysate

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

All lanes:

Western blot - Anti-Proteasome 20S C2/HC2 antibody (ab3325) at 2 µg/mL

Lane 1:

Untransfected Hep G2 whole cell extract.

Lane 2:

Proteasome 20S C2/HC2 non-targeting scrambled siRNA transfected Hep G2 whole cell extract.

Lane 3:

Proteasome 20S C2/HC2 knockdown Hep G2 whole cell extract.

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 30 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Chinese hamster, Hamster, Dog, Rat, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human PSMA1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25786

Specificity

Detects proteasome 20S C2/HC2 subunit.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Proteasome 20S C2/HC2 also known as the proteasome subunit beta type-1 represents an important component of the proteasome complex. This protein performs proteolytic functions degrading ubiquitinated proteins into peptides. The proteasome 20S C2/HC2 has a molecular mass of approximately 23 kDa and is expressed in various tissues throughout the body including muscle and nerve tissues. It forms part of the 20S core particle of the proteasome complex and contributes to protein homeostasis.

Biological function summary

Proteasome 20S C2/HC2 plays a significant role in maintaining protein quality by participating in proteolytic degradation. It forms part of the larger 26S proteasome complex which assembles with regulatory particles for substrate recognition. This protein is essential for the breakdown and removal of oxidized or misfolded proteins thereby preventing the accumulation of potentially toxic aggregates within the cell. It ensures cellular regulation by processing proteins that control various aspects of cell cycle signaling and differentiation.

Pathways

The activity of the Proteasome 20S C2/HC2 integrates directly into the ubiquitin-proteasome pathway. This pathway regulates protein turnover and plays a vital role in cellular processes such as the cell cycle and apoptosis. The association with proteins such as ubiquitin and E3 ligases is critical for the marking of substrate proteins for degradation. This protein is also linked to the NF-kB signaling pathway interacting with proteins that modulate immune and inflammatory responses demonstrating its influence on broader regulatory networks.

Proteasome 20S C2/HC2 disruption has been identified in various pathological conditions. Abnormal function or expression levels relate to neurodegenerative disorders like Parkinson’s disease where inefficient protein degradation leads to protein aggregation. This protein also connects to tumor progression in cancers where alterations in the ubiquitin-proteasome system affect cell cycle regulation and apoptosis. The protein's interaction with tumor suppressors and oncogenes highlights its significance in cellular growth abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA1

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Renal physiology 326:F814-F826 PubMed38545647

2024

Regulation of the water channel aquaporin-2 by cullin E3 ubiquitin ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Sathish K Murali,James A McCormick,Robert A Fenton

Communications biology 6:1127 PubMed37935829

2023

Synaptic proteasome is inhibited in Alzheimer's disease models and associates with memory impairment in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Felipe C Ribeiro,Danielle Cozachenco,Luana Heimfarth,Juliana T S Fortuna,Guilherme B de Freitas,Jorge M de Sousa,Soniza V Alves-Leon,Renata E P Leite,Claudia K Suemoto,Lea T Grinberg,Fernanda G De Felice,Mychael V Lourenco,Sergio T Ferreira

Nature communications 14:4092 PubMed37433777

2023

Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Minji Kim,Remigiusz A Serwa,Lukasz Samluk,Ida Suppanz,Agata Kodroń,Tomasz M Stępkowski,Praveenraj Elancheliyan,Biniyam Tsegaye,Silke Oeljeklaus,Michal Wasilewski,Bettina Warscheid,Agnieszka Chacinska

Nature aging 3:546-566 PubMed37118550

2023

Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Ju Lee,Hafiza Alirzayeva,Seda Koyuncu,Amirabbas Rueber,Alireza Noormohammadi,David Vilchez

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22583 PubMed36197017

2022

Genetic deletion of the nuclear factor of activated T cells 5 in collecting duct principal cells causes nephrogenic diabetes insipidus.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Petrillo,Dmitry Chernyakov,Cristina Esteva-Font,Søren B Poulsen,Bayram Edemir,Robert A Fenton

Frontiers in physiology 13:971251 PubMed36160843

2022

The E3 ubiquitin-protein ligase Nedd4-2 regulates the sodium chloride cotransporter NCC but is not required for a potassium-induced reduction of NCC expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lena L Rosenbaek,Federica Petrillo,Miguel X van Bemmelen,Olivier Staub,Sathish K Murali,Robert A Fenton

Frontiers in molecular neuroscience 15:861873 PubMed35531068

2022

Scaffold Protein Lnx1 Stabilizes EphB Receptor Kinases for Synaptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Na Li,Si Chen,Nan-Jie Xu,Suya Sun,Jin-Jin Chen,Xian-Dong Liu

Cells 11: PubMed35011657

2021

Potassium Effects on NCC Are Attenuated during Inhibition of Cullin E3-Ubiquitin Ligases.

Applications

Unspecified application

Species

Unspecified reactive species

Sathish K Murali,Robert Little,Søren B Poulsen,Mohammed Z Ferdaus,David H Ellison,James A McCormick,Robert A Fenton

Journal of cellular physiology 236:5012-5021 PubMed33400289

2021

Uromodulin aggravates renal tubulointerstitial injury through activation of the complement pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Fei Pei,Qiaoling Sun,Fei Shen,Xiangdong Yang,Zhao Hu,Maojing Liu

Cancer chemotherapy and pharmacology 85:843-853 PubMed32232513

2020

Evaluating the immunoproteasome as a potential therapeutic target in cisplatin-resistant small cell and non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuaki Shoji,Eiki Kikuchi,Junko Kikuchi,Yuta Takashima,Megumi Furuta,Hirofumi Takahashi,Kosuke Tsuji,Makie Maeda,Ichiro Kinoshita,Hirotoshi Dosaka-Akita,Jun Sakakibara-Konishi,Satoshi Konno

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Proteasome 20S C2/HC2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

Western blot - Anti-Proteasome 20S C2/HC2 antibody (AB3325)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

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